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安全信息

SRP0237

Sigma-Aldrich

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

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别名:
PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

human

重组

expressed in baculovirus infected insect cells

检测方案

≥90% (SDS-PAGE)

形式

aqueous solution

比活

≥1.4 pmol/min-μg

分子量

131 kDa

包装

pkg of 20 μg

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... PIK3CG(5294)

一般描述

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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