SRP0236

PI3 kinase (p110d/p85a) Active human

Name: PI3 kinase (p110d/p85a) Active human
Brand: SIGMA

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

别名:

PI3-kinase p110 subunit delta, PIK3CD, Phosphoinositide-3-kinase, catalytic, delta, ptdIns-3-kinase subunit p110-δ

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关于此项目

NACRES:

NA.32

UNSPSC Code:

12352200

Specific activity:

≥1.1 pmol/min-μg

Assay:

≥80% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in baculovirus infected insect cells

Concentration:

>0.02 mg/mL

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产品名称

PI3 kinase (p110d/p85a) Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥1.1 pmol/min-μg

mol wt

146 kDa (p110δ)
83.5 kDa (p85α)

packaging

pkg of 20 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_005026 (p110delta)
XM_043865 (p85alpha)

UniProt accession no.

O00329

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3CD(5293)

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

Other Notes

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.