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生物来源
human
重组
expressed in baculovirus infected insect cells
方案
≥80% (SDS-PAGE)
表单
aqueous solution
比活
≥1.1 pmol/min-μg
分子量
146 kDa (p110δ)
83.5 kDa (p85α)
包装
pkg of 20 μg
浓度
>0.02 mg/mL
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... PIK3CD(5293)
一般描述
Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
警示用语:
Danger
危险声明
危险分类
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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