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安全信息

SRP0116

Sigma-Aldrich

Sirtuin 2 human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

别名:

SIR2L2, SIRT2, sir2-like 2

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

human

重组

expressed in E. coli

方案

≥80% (SDS-PAGE)

表单

aqueous solution

分子量

35.5 kDa

包装

pkg of 100 μg

储存条件

avoid repeated freeze/thaw cycles

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... SIRT2(22933)

一般描述

Silent information regulator 2 (Sir2) or sirtuin 2 is encoded by the gene mapped to human chromosome 19q13.2. It belongs to class Ib of sirtuin protein family. Sir2 is characterized with a catalytic domain containing two different domains that bind NAD and the acetyl-lysine substrate, respectively. Apart from this, it also constitutes variable NH2 and COOH-terminal domains involved in the regulation of subcellular localizations and catalytic activity. sirtuin 2 is mainly expressed in cytosol but its migration between cytosol and nucleus facilitates the deacetylation of both α-tubulin and histones.
Human Sirtuin 2, GenBank Accession No. NM_030593, amino acids 50-356 with C-terminal His tag, MW = 35.5kDa, expressed in Escherichia coli expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Silent information regulator 2 (Sir2) acts as a nicotine adenine dinucleotide (NAD) dependent deacetylase and is implicated in various biological processes such as regulation of transcriptional repression, recombination, the cell-division cycle, microtubule organization, and cellular responses to DNA-damaging agents. In addition, sirtuins also regulate the process involved in aging. Sir2 plays a vital role in mammalian metabolic homeostasis and is considered to be a potent therapeutic target for the treatment of type 2 diabetes. Sir2 has a crucial role in reduction of microglial activation and brain inflammation. Therefore, loss of Sir2 activity increases the risk of susceptibility to neurodegenerative diseases.

单位定义

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Emerging Role of Sirtuin 2 in the Regulation of Mammalian Metabolism.
Gomes P
Trends in Pharmacological Sciences, 36(11), 756-768 (2015)
Yi Shi et al.
eLife, 3, e02349-e02349 (2014-06-19)
Recent studies suggested an essential role for seryl-tRNA synthetase (SerRS) in vascular development. This role is specific to SerRS among all tRNA synthetases and is independent of its well-known aminoacylation function in protein synthesis. A unique nucleus-directing domain, added at
The NAD-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation.
Pais TF
The Embo Journal, 32(19), 2603-2616 (2013)
Ruwin Pandithage et al.
The Journal of cell biology, 180(5), 915-929 (2008-03-12)
Cyclin-dependent kinases (Cdks) fulfill key functions in many cellular processes, including cell cycle progression and cytoskeletal dynamics. A limited number of Cdk substrates have been identified with few demonstrated to be regulated by Cdk-dependent phosphorylation. We identify on protein expression
Sirtuins: Sir2-related NAD-dependent protein deacetylases.
North BJ and Verdin E.
Genome Biology, 5(5), 224-224 (2004)

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