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生物来源
human
重组
expressed in baculovirus infected insect cells
检测方案
≥50% (SDS-PAGE)
形式
aqueous solution
分子量
56 kDa
包装
pkg of 50 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... HDAC1(3065)
一般描述
Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system.
生化/生理作用
Useful for the study of enzyme kinetics, screening inhibitors.
单位定义
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Laura De Clerck et al.
Molecular omics, 17(6), 929-938 (2021-09-16)
Histone-based chromatin organization paved the way for eukaryotic genome complexity. Because of their key role in information management, the histone posttranslational modifications (hPTM), which mediate their function, have evolved into an alphabet that has more letters than there are amino
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