SRE0018
藤黄节杆菌裂解酶
Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein
别名:
溶壁酶 来源于藤黄节杆菌
产品名称
藤黄节杆菌裂解酶, Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein
color
light brown
biological source
bacterial (Arthrobacter luteus)
form
lyophilized powder
specific activity
≥2000 units/mg protein
composition
Protein, ≥20% biuret
concentration
20 % protein Biuret
Quality Level
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
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Biochem/physiol Actions
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
Other Notes
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
Application
For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).
General description
Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Louis Dacquay et al.
G3 (Bethesda, Md.), 7(6), 1799-1809 (2017-04-30)
Actively proliferating cells constantly monitor and readjust their metabolic pathways to ensure the replenishment of phospholipids necessary for membrane biogenesis and intracellular trafficking. In Saccharomyces cerevisiae, multiple studies have suggested that the lysine acetyltransferase complex NuA4 plays a role in
实验方案
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
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