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此商品 | 72622 | 69222 | 74549 |
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application(s) cleaning products | application(s) environmental | application(s) environmental | application(s) environmental |
format single component solution | format single component solution | format single component solution | format single component solution |
grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® |
product line TraceCERT® | product line TraceCERT® | product line TraceCERT® | product line TraceCERT® |
concentration 1000 mg/L lactate in water (nominal concentration) | concentration 1000 mg/L in water (nominal concentration) | concentration 1000 mg/L in water (nominal concentration) | concentration 1000 mg/L gluconate in water (nominal concentration) |
一般描述
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna。
Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
In addition, the lentiviral transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.
应用
法律信息
推荐
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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