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Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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安全信息

SHC003V

Sigma-Aldrich

MISSION®

Green fluorescent protein marker to monitor transduction efficiency

别名:

MISSION®, MISSION® TurboGFP™ Control Transduction Particles

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选择尺寸

5 G
¥880.73
25 G
¥4,173.76
100 G
¥8,121.25

¥880.73

国内现货，预计发货时间2025年4月27日详情

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选择尺寸

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5 G
¥880.73
25 G
¥4,173.76
100 G
¥8,121.25

About This Item

UNSPSC代码:
41106609
NACRES:
NA.51

¥880.73

国内现货，预计发货时间2025年4月27日详情

获取大包装报价索取更多信息

质量水平

100
200

产品线

MISSION®

浓度

≥1x106 VP/ml (via p24 assay)

技术

capture ELISA: 106 TU/mL using p24

运输

dry ice

储存温度

−70°C

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form

powder

form

powder

form

powder

form

solid

λmax

534, 395, 312 nm (lit.)

λmax

531, 314, 295 nm (lit.)

λmax

534, 393, 313 nm (lit.)

λmax

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

assay

95% (NMR)

assay

95% (NMR)

assay

95% (NMR)

assay

96%

mp

>300 °C

mp

196 °C

mp

250-260 °C

mp

85-90 °C

一般描述

当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.[1]

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

应用

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.[2][3]

法律信息

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

推荐

产品编号
说明
价格

SHC002

任务 ® pLKO.1-puro 非哺乳动物 shRNA 对照质粒 DNA, Targets no known mammalian genes

SHC001V

MISSION®pLKO.1-puro空载体对照转导颗粒, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ 阳性对照质粒 DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION®, Targets no known mammalian genes

SHC004

MISSION® 对照载体, shRNA sequence targeting tGFP

SHC004V

MISSION® TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012V

MISSION®, Contains a gene encoding TagRFP

SHC014V

MISSION®, Contains a gene encoding TurboGFP, under the control of the UbC promoter

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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    06072112MN
    09041810MN

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    商品

    Methods for Enhancing Lentiviral Transduction Efficiency

    Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

    实验方案

    慢病毒转导实验方案

    如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。

    Lentiviral Transduction Protocol

    Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

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