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一般描述
用于全转录组扩增(WTA)的SeqPlex™RNA扩增试剂盒(SEQXE)专用于对少量或降解/高度片段化的RNA（如来自福尔马林固定石蜡包埋（FFPE）组织样本的RNA ）进行下一代测序(NGS)。通过SeqPlex试剂盒可对这类RNA以及其它用于NGS的少量/高度片段化RDNA样品进行预扩增。它还有助于扩增从组织、培养细胞、福尔马林固定样品或血清中分离的非 polyA 尾 RNA，同时保持未扩增样品中发现的差异表达模式。
应用
SeqPlex™ RNA扩增试剂盒已用于扩增poly（A）筛选的RNA。
特点和优势
- 少量总 RNA 随机引物技术可扩增来自所有来源（包括 FFPE 和 RIP）的片段化或完整 RNA。
- 半简并文库引物设计，可实现更完整的转录组覆盖和高效引发。
- 测序前无需进行 DNA的片段化。
- 在 8 小时或更短的时间内扩增 ds-cDNA。
- 兼容Pacific Bioscience以外的其它所有下一代测序平台。
其他说明
SEQR-500RXN 按需生产。请联系我们获取更多信息。
法律信息
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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法规信息
常规特殊物品
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different
实验方案
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
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