SAE0092
变溶菌素 来源于球孢链霉菌 ATCC 21553
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
别名:
Mutanolysin
产品名称
变溶菌素 来源于球孢链霉菌 ATCC 21553, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
biological source
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
~18.5 kDa
feature
DNA free
concentration
≥40% protein
technique(s)
nucleic acid detection: suitable
suitability
suitable for cell lysis
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
纯化的变溶菌素SAE0092已经过严格的质量控制测试，以确保通过35个循环对16S和18S rDNA（使用通用引物对）进行PCR扩增时，不存在可检测水平的污染DNA。
变溶菌素是一种胞壁溶解酶，适合分离来自革兰氏阳性菌（如李斯特菌、乳杆菌、乳球菌）(8)和其他仅用溶菌酶难以裂解的细菌。
变溶菌素是一种胞壁溶解酶，适合分离来自革兰氏阳性菌（如李斯特菌、乳杆菌、乳球菌）(8)和其他仅用溶菌酶难以裂解的细菌。
Biochem/physiol Actions
变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
Features and Benefits
适用于难以裂解的样品。
无微生物DNA，最大限度地减少工作流程中试剂对DNA的污染。
适用于从革兰氏阳性菌中分离核酸，包括李斯特菌、乳酸杆菌和乳球菌。
无微生物DNA，最大限度地减少工作流程中试剂对DNA的污染。
适用于从革兰氏阳性菌中分离核酸，包括李斯特菌、乳酸杆菌和乳球菌。
Other Notes
以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600。
Physical form
冻干粉，含Ficoll® 和琥珀酸钠缓冲盐
Legal Information
Ficoll is a registered trademark of Cytiva
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Jennifer A Taylor et al.
Current protocols, 1(4), e80-e80 (2021-04-13)
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2-alkyne muramic acid
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
商品
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程：只需加入酶，将试管放入水浴然即可。但这过程中到底发生了什么？
相关内容
Instructions
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