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Merck
CN

SAE0091

Sigma-Aldrich

溶葡球菌酶 来源于溶葡萄球菌

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

别名:

溶葡球菌酶 来源于溶葡萄球菌, 甘氨酰-甘氨酸内肽酶

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.54

product name

溶葡球菌酶 来源于溶葡萄球菌, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

生物来源

microbial (Staphylococcus staphylotyticus)

形式

lyophilized powder

比活

≥500 units/mg protein

分子量

27 kDa

特点

DNA free

运输

wet ice

储存温度

−20°C

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应用

从葡萄球菌SAE0091中纯化的细胞外溶葡萄球菌素已经过严格的质量控制测试,以确保通过35个循环对16S和18S rDNA(使用通用引物对)进行PCR扩增时,不存在可检测水平的污染DNA。
溶葡萄球菌酶是从溶葡萄球菌的细菌培养物中分离出的锌金属酶。它对其他葡萄球菌(包括金黄色葡萄球菌)具有特异性裂解作用。1,2溶葡萄球菌素具有
己糖胺酶、酰胺酶和内肽酶活性。它裂解细胞壁中的聚甘氨酸交联,导致葡萄球菌物种的细胞裂解,但不导致其他细菌属的细胞裂解。[Pharmaceuticals 2010, 3(4), 1139-1161]
溶葡萄球菌素是一条包含246个氨基酸的多肽链,分子量为26,926 Da,等电点为9.5,(5),最适pH值为7.5。(6)

生化/生理作用

溶葡萄球菌素是一种分子量约为25 kDa的锌内肽酶。由于溶葡萄球菌酶能裂解 葡萄球菌 类细胞壁肽聚糖层中的聚甘氨酸交联键,它已被发现可用于细胞裂解,并具有潜在的抗菌治疗作用。活性的最优pH值:~7.5。
溶葡萄球菌素是一种分子量约为25 kDa的锌内肽酶。 由于溶葡萄球菌酶能裂解 葡萄球菌 类细胞壁肽聚糖层中的聚甘氨酸交联键,它已被发现可用于细胞裂解,并具有潜在的抗菌治疗作用。
活性最佳pH:约 7.5

单位定义

一个单位在25℃和pH 7.5条件下,在6.0 ml反应混合物中可使金黄色葡萄球菌细胞混悬液的浊度(A620)在10分钟内由0.250降至0.125。

外形

以冻干粉形式提供,其中含有50-70%的蛋白质,其余主要为NaCl。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Ronald M Galiwango et al.
Microbiome, 10(1), 7-7 (2022-01-20)
Coronal sulcus (CS) anaerobe abundance and IL-8 levels are linked to HIV acquisition, and are dramatically reduced after penile circumcision (PC). The distal urethra may be the site of some HIV acquisition before PC, and presumably most acquisition post PC. We
Kojiro Tsujihana et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2116027119-e2116027119 (2022-06-16)
The epidermis is the outermost layer of the skin and the body's primary barrier to external pathogens; however, the early epidermal immune response remains to be mechanistically understood. We show that the chemokine CXCL14, produced by epidermal keratinocytes, exhibits robust
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin
Astrid Haaskjold Lossius et al.
Dermatology (Basel, Switzerland), 238(1), 109-120 (2021-04-23)
The pathophysiology in atopic dermatitis (AD) is not fully understood, but immune dysfunction, skin barrier defects, and alterations of the skin microbiota are thought to play important roles. AD skin is frequently colonized with Staphylococcus aureus (S. aureus) and microbial

商品

裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程:只需加入酶,将试管放入水浴然即可。但这过程中到底发生了什么?

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

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