生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
NOQ7.5.4D, monoclonal
形式
buffered aqueous solution
种属反应性
sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine
包装
antibody small pack of 25 μL
浓度
~1 mg/mL
技术
immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate.
immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system.
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MYH7(4625)
一般描述
单克隆抗肌球蛋白（骨骼肌，慢肌）（小鼠IgG1同种型）来源于小鼠骨髓瘤细胞和BALB / c小鼠脾细胞融合产生的NOQ7.5.4D杂交瘤。肌球蛋白是一种六聚体蛋白，包含两个重链亚基和四个轻链亚基。它是心脏和骨骼肌的主要蛋白质成分。肌肉肌球蛋白重链（MHC）的不同同种型根据肌肉的生理功能进行表达：骨骼、心脏、平滑和非肌肉。
免疫原
肌球蛋白，从人骨骼肌分离的肌原纤维中提纯，
应用
小鼠生产的单克隆抗肌球蛋白（骨骼肌，慢肌）抗体已被用于免疫印迹、免疫组织学、固相放射免疫测定（RIA）、酶联免疫吸附测定（ELISA）和免疫电镜检查。
生化/生理作用
肌球蛋白是肌动蛋白依赖性马达。肌球蛋白基因突变与肌病、失明和听力损失有关。它们参与肌肉收缩、视觉、听觉、细胞运动和细胞侵袭。
肌球蛋白负责心脏和骨骼肌的能量传导和力量发展（force development）。现已确知肌球蛋白可与肌肉和非肌肉细胞中的肌动蛋白相互作用。慢肌球蛋白，也称β-肌球蛋白重链（β-MHC），在胚胎发生过程中表达，它是心脏生肌程序（cardiac myogenic program）的一部分，受到NK-2同源框蛋白5（NKX-2.5）、肌细胞增强因子2C（MEF- 2C）和GATA-4 / 5/6的控制。
外形
溶于 0.01 M 磷酸盐缓冲液 (pH 7.4) 中，含 15 mM 叠氮化钠 e。
法律信息
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Bernardo J Foth et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(10), 3681-3686 (2006-03-01)
Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites. Myosin heavy chains consist of distinct head, neck, and tail domains and have
Jelle C B C de Jong et al.
Biology of sex differences, 14(1), 45-45 (2023-07-11)
Physical weakness is a key component of frailty, and is highly prevalent in older adults. While females have a higher prevalence and earlier onset, sex differences in the development of frailty-related physical weakness are hardly studied. Therefore, we investigated the
Jelle C B C de Jong et al.
GeroScience, 46(3), 3341-3360 (2024-01-24)
Muscle-aging drives sarcopenia and is a major public health issue. Mice are frequently used as a model for human muscle-aging, however, research investigating their translational value is limited. In addition, mechanisms underlying muscle-aging may have sex-specific features in humans, but
Heavy and light roles: myosin in the morphogenesis of the heart
England J and Loughna S
Cellular and Molecular Life Sciences, 70(7), 1221-1239 (2013)
Caloric Restriction Combined with Immobilization as Translational Model for Sarcopenia Expressing Key-Pathways of Human Pathology.
de Jong, et al.
Aging and disease, 14, 937-957 (2023)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门