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产品名称
抗-LC3B抗体，小鼠单克隆, clone LC3B-6, purified from hybridoma cell culture
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
LC3B-6, monoclonal
表单
buffered aqueous solution
分子量
antigen ~18/16 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B.
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAP1LC3B(81631)
一般描述
MAP1LC3B基因（微管相关蛋白1轻链3β）是酵母中的自噬相关基因ATG8的一种同源基因。编码的蛋白质称为pro-MAP1LC3B，这是一种被ATG4裂解以产生MAP1LC3B-1胞浆形式的前体形式。 小鼠单克隆抗LC3B抗体（小鼠IgG2b同种型）来源于LC3B-6杂交瘤，该杂交瘤是由小鼠骨髓瘤细胞与用对应于人LC3B N末端区域的合成肽免疫的BALB / c小鼠脾细胞融合产生。
免疫原
对应于人LC3B的N-末端区域的合成肽，与KLH耦合。相应的序列在大鼠和小鼠中相差一个氨基酸。
生化/生理作用
在自噬过程中被插入至生长中的自噬囊泡的内膜和外膜前，MAP1LC3B-I（微管相关蛋白1轻链3β-I）会结合MAP1LC3B-II。MAP1LC3B的细胞分布和加工有助于对自噬进行监测。缺乏MAP1LC3B的细胞被发现在自噬囊泡的形成中存在缺陷，其改变的表型与自噬在能量稳态和蛋白聚集体去除中的作用相关。它可作为一种自噬的标志物。
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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