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Merck
CN
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文件

S0266

Sigma-Aldrich

精脒

BioReagent, for molecular biology, suitable for cell culture, ≥98%

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别名:
1,8-二氨基-4-氮杂辛烷, N-(3-氨基丙基)-1,4-丁烷
线性分子式:
NH2(CH2)3NH(CH2)4NH2
CAS号:
124-20-9
分子量:
145.25
Beilstein:
1698591
EC 号:
204-689-0
MDL编号:
MFCD00008229
UNSPSC代码:
12352200
PubChem化学物质编号:
24899434
NACRES:
NA.52

等级

for molecular biology

质量水平

200

产品线

BioReagent

检测方案

≥98%

形式

solid or liquid

分子量

145.25 (AMU)

技术

cell culture | mammalian: suitable

折射率

n20/D 1.479 (lit.)

密度

0.925 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, and protease, none detected

储存温度

room temp

SMILES字符串

NCCCCNCCCN

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

基因信息

rat ... Ppm1a(24666)

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一般描述

亚精胺属于天然多胺，存在于叶绿体、细菌或真核细胞中。

应用

亚精胺可用于制备金珠并用作核分离缓冲液（NIB）成分。
精胺适合用于分子生物缓冲液和> 100 bp DNA沉淀。它被用来在植物和哺乳动物细胞中，通过粒子轰击技术，传递DNA。

生化/生理作用

亚精胺有助于维持基因表达的正常活性。此多胺在中间代谢中起关键作用。也可用作E1A相关蛋白p300（EP300）乙酰转移酶活性的抑制剂。亚精胺参与细胞生长。
抑制神经元型一氧化氮合成酶（nNOS）。结合和沉淀DNA，可用于DNA结合蛋白纯化。激活T4聚核苷酸激酶活性。

注意

如果需要无菌溶液，则应采用无菌过滤而非高压灭菌。时间一长，亚精胺将发生脱氨基作用；溶液应采用冷冻保存。通常需要配制新溶液。

相关产品

产品编号
说明
价格

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H314

危险分类

Eye Dam. 1 - Skin Corr. 1B

WGK

WGK 3

闪点(°F)

233.6 °F

闪点(°C)

112 °C

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is this a spermidine solution or a neat liquid, and what is the concentration?

    Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Organization, developmental dynamics, and evolution of plastid nucleoids
Sato N, et al.
International Review of Cytology, 232, 217-262 (2003)
Molecular Determinants of Metaplastic and Neoplastic Transformation in the Esophageal Epithelium
Progress in Molecular Biology and Translational Science, 96, 63-92 (2010)
Spermidine delays aging in humans
Madeo F, et al.
Aging (Albany. NY.), 10(8), 2209-2209 (2018)
Elumalai Sivamani et al.
Plant cell reports, 28(2), 213-221 (2008-11-19)
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded
Delivery of DNA to skin by particle bombardment.
Shixia Wang et al.
Methods in molecular biology (Clifton, N.J.), 245, 185-196 (2004-01-07)
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