推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 160 kDa
种属反应性
human, rat, mouse
技术
immunoprecipitation (IP): 10-20 μL using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
microarray: suitable
western blot: 1:1,000-1:2,000 using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ROCK1(6093)
mouse ... Rock1(19877)
rat ... Rock1(81762)
一般描述
Rho-associated coiled-coil protein kinase 1 or ROCK-1 is a serine/threonine kinase that regulates actin assembly, cell movement and contraction. This kinase activates the JNK signaling pathway, modulates stress granule formation and regulates apoptosis in cardiac myocytes and stress granules .
免疫原
synthetic peptide corresponding to amino acids 1336-1354 located at the C-terminus of human ROCK-1, conjugated to KLH. This sequence is identical in mouse and rat ROCK-1.
应用
Anti-ROCK-1 antibody produced in rabbit has been used in western blotting and immunocytochemistry.
生化/生理作用
Rho-associated kinases (ROCK or Rho-kinase) is implicated in Rho-mediated actin reorganization such as formation of stress fibres and focal adhesions and smooth muscle contraction. Rho-kinases lead to an increase of myosin light chain (MLC) phosphorylation and thereby induce actomyosin based contractility. ROCK accumulates at the cleavage furrow during cytokinesis. In addition, a dominant negative form of ROCK increases multinuclei in mammalian cells. ROCK directly phosphorylates and activates LIM-kinase (LIMK), resulting in downstream phosphorylation and inaction of the actin depolymerizing factor or cofilin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Rho plays a key role in TGF-beta1-induced proliferation and cytoskeleton rearrangement of human periodontal ligament cells
Wang L, et al.
Archives of Oral Biology, 59(2), 149-157 (2014)
Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions
Katoh K, et al.
Journal of the Royal Society, Interface / the Royal Society, 8(56), 305-305 (2011)
Marc Jacobs et al.
The Journal of biological chemistry, 281(1), 260-268 (2005-10-27)
ROCK or Rho-associated kinase, a serine/threonine kinase, is an effector of Rho-dependent signaling and is involved in actin-cytoskeleton assembly and cell motility and contraction. The ROCK protein consists of several domains: an N-terminal region, a kinase catalytic domain, a coiled-coil
Pat P Ongusaha et al.
Science signaling, 1(47), ra14-ra14 (2008-11-28)
Although apoptosis triggered by ultraviolet B (UVB)-mediated activation of the c-Jun N-terminal kinase (JNK) pathway is mediated by both intrinsic and extrinsic pathways, the mechanism of initiation of JNK activation remains obscure. Here, we report the characterization of the JNK-interacting
T Sumi et al.
The Journal of biological chemistry, 276(1), 670-676 (2000-10-06)
LIM-kinase 1 (LIMK1) and LIM-kinase 2 (LIMK2) regulate actin cytoskeletal reorganization via cofilin phosphorylation downstream of distinct Rho family GTPases. We report our findings that ROCK, a downstream protein kinase of Rho, specifically activates LIMK2 but not LIMK1 downstream of
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