form
solution
Quality Level
usage
sufficient for ≥600 reactions
feature
PCR Additives
concentration
100 ×
technique(s)
PCR: suitable
color
red
input
crude DNA
solubility
water: soluble
storage temp.
2-8°C
General description
Sigma′定量聚合酶链反应（qPCR）参考染料是一种专有染料，用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时，它可以用于反应数据的标准化。 我们提供100×参考染料，最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。
Application
定量 PCR 参考染料已被用于:
- 制备用于实时定量聚合酶链反应 （RT-qPCR）的预混液
- 作为反应混合物的成分，用于通过定量聚合酶链式反应（qPCR）检测艰难梭菌
- 通过 qPCR 分析细胞 DNA 污染程度
Other Notes
定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。
signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
ppe
Eyeshields, Gloves
实验方案
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R4526-5ML | 04061837693939 |
| R4526-20RXN | 04061832933283 |
| R4526-.3ML | 04061836695354 |