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Merck
CN

R4526

定量PCR参考染料

100 ×, solution

别名:

qPCR参考染料, ROX

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106300
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产品名称

定量PCR参考染料, 100 ×, solution

form

solution

usage

sufficient for ≥600 reactions

concentration

100 ×

technique(s)

PCR: suitable

color

red

solubility

water: soluble

storage temp.

2-8°C

Quality Level

Application

定量 PCR 参考染料已被用于:
  • 制备用于实时定量聚合酶链反应 (RT-qPCR)的预混液
  • 作为反应混合物的成分,用于通过定量聚合酶链式反应(qPCR)检测艰难梭菌
  • 通过 qPCR 分析细胞 DNA 污染程度

General description

Sigma′定量聚合酶链反应(qPCR)参考染料是一种专有染料,用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时,它可以用于反应数据的标准化。 我们提供100×参考染料,最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。

Other Notes

定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

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Ryan Manow et al.
Journal of industrial microbiology & biotechnology, 39(7), 977-985 (2012-03-01)
Previously, a native homoethanol pathway was engineered in Escherichia coli B by deletions of competing pathway genes and anaerobic expression of pyruvate dehydrogenase (PDH encoded by aceEF-lpd). The resulting ethanol pathway involves glycolysis, PDH, and alcohol dehydrogenase (AdhE). The E.
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Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
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Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
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Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)

商品

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

实验方案

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。

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