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一般描述
Sigma′定量聚合酶链反应（qPCR）参考染料是一种专有染料，用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时，它可以用于反应数据的标准化。 我们提供100×参考染料，最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。
应用
定量 PCR 参考染料已被用于:
- 制备用于实时定量聚合酶链反应 （RT-qPCR）的预混液
- 作为反应混合物的成分，用于通过定量聚合酶链式反应（qPCR）检测艰难梭菌
- 通过 qPCR 分析细胞 DNA 污染程度
其他说明
定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。
警示用语：
Warning
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Ryan Manow et al.
Journal of industrial microbiology & biotechnology, 39(7), 977-985 (2012-03-01)
Previously, a native homoethanol pathway was engineered in Escherichia coli B by deletions of competing pathway genes and anaerobic expression of pyruvate dehydrogenase (PDH encoded by aceEF-lpd). The resulting ethanol pathway involves glycolysis, PDH, and alcohol dehydrogenase (AdhE). The E.
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
Gustav Johansson et al.
Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
商品
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
实验方案
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
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