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安全信息

PLN70

Sigma-Aldrich

GenElute™质粒小量制备试剂盒

greener alternative

sufficient for 70 purifications

别名:

GenElute™质粒试剂盒, Gen Elute

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.52
价格与库存信息目前不能提供

用途

sufficient for 70 purifications

质量水平

200

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

DNA purification: suitable

环保替代产品分类

, Aligned

储存温度

15-25°C

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此商品
RAB0322RAB0250RAB0305
人IL-10 R β ELISA试剂盒 for serum, plasma, cell culture supernatant and urine

RAB0249

人IL-10 R β ELISA试剂盒

人类 IL-9 ELISA 试剂盒 for serum, plasma, cell culture supernatant and urine

RAB0322

人类 IL-9 ELISA 试剂盒

Human IL-11 ELISA Kit for serum, plasma, cell culture supernatant and urine

RAB0250

Human IL-11 ELISA Kit

人类IL-5 R α ELISA试剂盒 for serum, plasma, cell culture supernatant and urine

RAB0305

人类IL-5 R α ELISA试剂盒

Gene Information

human ... IL10RB(3588)

Gene Information

human ... IL9(3578)

Gene Information

human ... IL11(3589)

Gene Information

human ... IL5RA(3568)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.12-3000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.04-30 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 3.29-800 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.164-40 ng/mL

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

一般描述

GenElute 质粒小量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒结合了硅胶结合技术和离心柱形式的便利性，可在30分钟内从1-5 mL大肠杆菌培养物中回收出至多15 μg高拷贝数质粒DNA。请注意，实际得率和最佳的培养物体积取决于质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。

应用

此类DNA可直接用于限制性酶切、克隆、PCR、转化、转录、传统和自动化测序等下游应用。

特点和优势

  • 可在30分钟内获得纯化的质粒DNA
  • 至多可纯化出15 μg质粒DNA
  • 检测不到基因组DNA或RNA污染
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

其他说明

更多信息，请见www.sigma-aldrich.com/genelutehp。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Central nervous system

储存分类代码

3 - Flammable liquids

法规信息

危险化学品
动植物源性产品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000434404
    0000434404
    SLBL5378V
    0000386901
    0000386901

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    Timothy A Scott et al.
    Cell, 169(3), 442-456 (2017-04-22)
    Fluoropyrimidines are the first-line treatment for colorectal cancer, but their efficacy is highly variable between patients. We queried whether gut microbes, a known source of inter-individual variability, impacted drug efficacy. Combining two tractable genetic models, the bacterium E. coli and the nematode C. elegans
    Cloning and Characterization of Serine-rich Entamoeba histolytica Protein Gene from an Iranian E. histolytica Isolate
    Sima Rasti
    Pakistan journal of biological sciences: PJBS, 9, 654-658 (2006)
    Genetic determinants of resistance to fusidic acid among clinical bacteremia isolates of Staphylococcus aureus.
    Lannergard J
    Antimicrobial Agents and Chemotherapy, 53(5), 2059-2065 (2009)
    Comparison of three molecular methods for the detection of pilchard herpesvirus in archived paraffin-embedded tissue and frozen tissue.
    Crockford M
    Diseases of Aquatic Organisms, 82(1), 37-44 (2008)
    Ky V Hoang et al.
    Frontiers in microbiology, 8, 1695-1695 (2017-09-29)
    Francisella tularensis (F. tularensis) is the causative agent of tularemia and is classified as a Tier 1 select agent. No licensed vaccine is currently available in the United States and treatment of tularemia is confined to few antibiotics. In this
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    实验方案

    Escort™ III Transfection Reagent Protocol

    The product bulletin providin detailed use protocol for easy DNA transfection.

    Escort™ IV Transfection Reagent Protocol

    Product manual provides detailed protocol for easy DNA transfection.

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