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安全信息

PCRISPR004

Sigma-Aldrich

Mouse CRISPR Brie Knockout Library

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NACRES:
NE.02

包装

pkg of 5 vials (5x200µL aliquots )

浓度

≥5x108 VP/ml (via p24 Assay)

应用

CRISPR

运输

dry ice

储存温度

−70°C

一般描述

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

  • Functional Genomics/Target Validation
  • Unbiased wholed genome forward genetic screening
  • Validated positive and negative controls
  • Set up and optimization of screen assay

特点和优势

  • Focus on your research, and we will generate your lentivirus screening library.
  • Use CRISPR nucleases to knockout protein-coding genes to assess their function.
  • Mouse Genes targeted 19,674
  • Compact library of ~ four gRNAs per gene (78,637 total)
  • Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.

原理

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

法律信息

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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John G Doench et al.
Nature biotechnology, 34(2), 184-191 (2016-01-19)
CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target

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