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安全信息

P9620

Sigma-Aldrich

嘌呤霉素

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

别名:

3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride

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About This Item

CAS号:
58-58-2
MDL编号:
MFCD00012691
UNSPSC代码:
51281906
PubChem化学物质编号:
329820352
NACRES:
NA.85

生物来源

Streptomyces alboniger

质量水平

300

类型

for cell culture

检测方案

>98% (HPLC)

形式

solution

储存条件

(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )

浓度

10 mg/mL in H2O

技术

cell culture | mammalian: suitable

颜色

colorless to yellow

溶解性

H2O: soluble 10 mg/mL

抗生素抗菌谱

Gram-positive bacteria
neoplastics
parasites

作用机制

protein synthesis | interferes

运输

wet ice

储存温度

−20°C

SMILES字符串

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChI key

MKSVFGKWZLUTTO-FZFAUISWSA-N

相关类别

一般描述

嘌呤霉素，也称杆菌肽或嘌啉霉素盐酸盐，是一种氨基核酸酶抗生素和蛋白质合成抑制剂。它是由土壤放线菌白黑链霉菌（Streptomyces alboniger）通过一系列酶促反应合成。嘌呤霉素有效抑制原核和真核细胞，其作用原理是干扰RNA功能，从而抑制蛋白质合成。它也充当氨基肽酶和脑啡肽酶的抑制剂。嘌呤霉素可以使细菌、原生动物、水藻和哺乳动物细胞的生长迅速停止，但是含pac基因的细胞有耐药性。 pac基因的基因产物是嘌呤霉素-N-乙酰基转移酶，这种酶通过乙酰化反应使嘌呤霉素失活。

嘌呤霉素与tRNA的含氨基末端结构相似，这一特性令其在蛋白质合成过程中进入核糖体，结合新生多肽链，并使链的延长停止。嘌呤霉素广泛用于细胞培养，可作为以耐嘌呤霉素基因转化的细胞的选择剂。

应用

嘌呤霉素是一种氨基核苷酸抗生素，衍生自白黑链霉菌。 它可以选择包含抗性基因嘌呤霉素 N-乙酰基转移酶（PAC）的细胞。 它被用在兔模型中，研究处理后的血管平滑肌细胞的生存能力。 嘌呤霉素用在体细胞克隆和胚胎移植后产生增强的绿色荧光蛋白（EGFP）转基因仔猪。

生化/生理作用

作用机制：嘌呤霉素通过提前终止链来抑制蛋白质合成，作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。嘌呤霉素还可作为二肽基肽酶 II（丝氨酸肽酶）和胞质溶胶丙氨酰氨基肽酶的可逆抑制剂起作用。

耐药性机制：嘌呤霉素乙酰转移酶是有效的抗性基因。

抗菌谱：该产品对革兰氏阳性微生物有活性，对耐酸杆菌的活性较低，对革兰氏阴性微生物的活性更低。 嘌呤霉素可以防止细菌、原生动物、藻类和哺乳动物细胞的生长，并且起效迅速，可在 2 天内杀死 99％ 的细胞。

特点和优势

  • 适用于多种研究应用的优质抗生素
  • 抗生素和蛋白质合成抑制剂
  • 有效抑制原核和真核细胞
  • 通过干扰RNA功能抑制蛋白质合成
  • 常用作细胞生物学研究中的选择剂

制备说明

将该产物以 10 mg/mL 的浓度浓缩在水中。已在转染了 pac 耐药基因的 HeLa 细胞上测试了其生长抑制和细胞选择的能力。

其他说明

保存于密闭容器内，置于干燥通风处。
如需了解生化试剂系列的更多信息，请填写此表。

可比产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

监管及禁止进口产品

分析证书(COA)

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S Eyckerman et al.
Nature cell biology, 3(12), 1114-1119 (2002-01-10)
Ligand-induced clustering of type I cytokine receptor subunits leads to trans-phosphorylation and activation of associated cytosolic janus kinases (JAKs). In turn, JAKs phosphorylate tyrosine residues in the receptor tails, leading to recruitment and activation of signalling molecules. Among these, signal
Ming Lee Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(8), 3029-3034 (2008-02-15)
Cross-presentation as a fundamental pathway of activating CD8(+) T cells has been well established. So far the application of this concept in vivo is limited, and the mechanisms that specialize CD8(+) dendritic cells (DCs) for this task are not fully
Valerie Croons et al.
The Journal of pharmacology and experimental therapeutics, 325(3), 824-832 (2008-03-07)
Recent evidence indicates that the protein synthesis inhibitor cycloheximide triggers selective macrophage death in rabbit atheroma-like lesions without affecting smooth muscle cells (SMCs) or the endothelium, thereby favoring a stable plaque phenotype. In this study, we report that puromycin, a
Satoshi Watanabe et al.
Biology of reproduction, 72(2), 309-315 (2004-09-24)
Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic puromycin (an effective inhibitor of protein synthesis), has been widely used as a dominant selection marker in embryonic stem (ES) cell-mediated transgenesis. The present study is the first
Luca L Fava et al.
Genes & development, 31(1), 34-45 (2017-01-29)
Centrosomes, the main microtubule-organizing centers in animal cells, are replicated exactly once during the cell division cycle to form the poles of the mitotic spindle. Supernumerary centrosomes can lead to aberrant cell division and have been causally linked to chromosomal
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