一般描述
常见工作浓度：在植物组织培养基中1.5-2.5 g/L；在微生物培养基中最高10 g/L。Phytagel™需要阳离子（特别是二价）用于凝胶的发生。大多数植物组织培养基中通常含有的钙和镁浓度足以实现凝胶化。低盐培养基配方，特别是那些用于微生物应用的可能需要补充额外的钙或镁盐（如CaCl2或MgSO4）或更高浓度的Phytagel。
应用
Phytagel™已用于：
- 拟南芥中根检测的培养基固化
- 作为农杆菌培养中固体Paul′s培养基的一种成分
- 冬青属组织培养中根诱导培养基的一种组分
生化/生理作用
Phytagel旨在用于代替琼脂和其他用于植物组织培养的凝胶剂。Phytagel已被证明是香蕉幼苗微繁殖中组织培养级琼脂的优质替代品。
其他说明
一种由葡萄糖醛酸、鼠李糖和葡萄糖组成的细菌基质产生的琼脂替代品. 它能产生透明、无色、高强度的凝胶，有助于微生物污染的检测。
重悬
为防止结块，应在加热前缓慢的加入到快速搅拌的培养基中。
法律信息
Phytagel is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
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CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
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Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
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Applied and environmental microbiology, 75(24), 7752-7759 (2009-10-27)
In the context of an increasing utilization of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species.
实验方案
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
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