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表单
ammonium sulfate suspension
质量水平
比活
≥600 units/mg protein
UniProt登记号
储存温度
2-8°C
基因信息
pig ... PLA2G1B(445525)
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一般描述
磷脂酶 A2 是一种富含二硫键的小分子蛋白，有 124 个残基。 是一种钙依赖性酶。
应用
磷脂酶 A2 已用于磷脂酶试验，并确定大鼠肾近曲小管段 (PTS) 在氧合和缺氧-复氧过程中的活性。
生化/生理作用
与单体底物相比，它对聚集的底物具有较高的催化活性。
水解 β-两性甘油磷脂的酯键。首选底物为磷脂酰胆碱、磷脂酰乙醇胺及其缩醛磷脂类似物。磷脂酰肌醇和磷脂酰丝氨酸也被水解。其会攻击完整细胞膜上的磷脂。
单位定义
在 pH 8.0、37°C 条件下，1 个单位每分钟将 1.0 μmol 大豆 L-α-磷脂酰胆碱水解为 L-α-溶血磷脂酰胆碱和 1 个脂肪酸。
外形
在 3.2 M (NH 4 ) 2 SO 4 溶液 (pH 5.5) 中的混悬液
分析说明
双缩脲法测定蛋白质。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Knut Kölbel et al.
Biophysical chemistry, 206, 12-21 (2015-06-29)
Porcine pancreatic phospholipase A2, a small and disulfide rich protein, is extremely resistant against chemically or thermally induced unfolding. Despite this marked resistance, the protein displays broad unfolding transitions resulting in comparatively low apparent thermodynamic stability. Broad unfolding transitions may
R A Zager et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8297-8301 (1993-09-01)
During hypoxic or ischemic renal tubular injury, phospholipase A2 (PLA2) induces membrane deacylation, causing fatty acid accumulation and phospholipid breakdown. Because these changes can compromise cellular integrity, PLA2 activity has been widely proposed as a critical mediator of hypoxic renal
B van den Berg et al.
The EMBO journal, 14(17), 4123-4131 (1995-09-01)
The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo. The enzyme has very high catalytic activities on aggregated substrates compared with monomeric substrates, a phenomenon called interfacial activation. Crystal structures of
B van den Berg et al.
Journal of biomolecular NMR, 5(2), 110-121 (1995-02-01)
The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy. The structure of the protein (124 residues) is based on 1854
Elena Venuti et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 16(6), 763-770 (2017-07-26)
Bile salt stimulated lipase (BSSL; Enzyme Commission (EC) number 3.1.1.13) has been a candidate triglyceridase for improving enzyme therapy for pancreatic insufficiency; however, its efficacy is near absent. We hypothesise that similarly to pancreatic lipase, BSSL is inhibited by phospholipids
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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