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Merck
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文件

P6407

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

别名:

PDL

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About This Item

线性分子式:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS号:
27964-99-4
MDL编号:
MFCD00131934
UNSPSC代码:
12352202
PubChem化学物质编号:
24898764
NACRES:
NA.75

product name

聚-D-赖氨酸 氢溴酸盐, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

生物来源

synthetic (organic)

质量水平

200

无菌性

γ-irradiated

产品线

BioReagent
BioXtra

形式

lyophilized powder

分子量

70,000-150,000

包装

pkg of 10 X 5 mg
pkg of 5 mg

浓度

0.016—0.032 mmol lysine

技术

cell culture | mammalian: suitable

表面覆盖度

4 μg/cm2

杂质

<10% water (by Karl Fischer)

溶解性

H2O: soluble 50 mg/mL, clear, colorless

运输

ambient

储存温度

−20°C

SMILES字符串

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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应用

多聚-D-赖氨酸氢溴酸盐已用于:

  • 作为培养板的涂层用于培养大鼠神经胶质前体细胞(RGPCS)。
  • 用于包被继代培养伸长细胞的培养瓶和培养板
  • 处理盖玻片的表面以便细胞附着
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。

建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。

生化/生理作用

多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。

特点和优势

  • 用伽马（γ）射线消毒
  • 在2-8˚C下无菌多聚-D-赖氨酸溶液可保存三年。

组分

聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

注意

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

制备说明

该产品具有70,000-150,000的分子量并经细胞培养检测和γ射线照射。如需去除HBr，将该产品溶解于中性缓冲液并通过透析以去除这些盐。 通常，如需将该产品作为附着因子使用，可将50 mL无菌组织培养级用水加入5 mg的聚赖氨酸中，并用1 mL溶液对每25 cm2的表面进行无菌涂覆。 在5分钟后，通过吸液将溶液去除并彻底冲洗表面。 在加入细胞和培养基前让其干燥两小时。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Do you have a protocol for coating cell cultureware or glass coverslips/slides with Product P6407, Poly-D-Lysine?

    The protocol for coating surfaces with poly-D-lysine is the same as for poly-L-lysine.  The optimal conditions must be determined for each cell line and application. In general, the following steps can be used.1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-D-lysine.2. Aseptically coat culture surface with 0.5-1.0 ml of solution per 25 cm2. Rock gently to ensure even coating of the culture surface.3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.4. Allow to dry at least two hours before introducing cells and medium.

  3. Does Product P6407, Poly-D-lysine hydrobromide, need to be dialyzed to remove the hydrogen bromide?

    The hydrogen bromide will not interfere with the cell growth.  The hydrogen bromide salts are used to make our poly-L-lysine solutions that are cell culture tested.  For these products, there is no interference in cell growth from the bromide.

  4. Why would I use Product P6407, Poly-D-lysine hydrobromide, instead of poly-L-lysine?

    Certain cells can digest poly-L-lysine, in which case the poly-D-lysine should be used.

  5. How do I determine which molecular weight range of poly-lysine to use?

    Poly-lysine is used to coat surfaces to increase the charge present. The lower molecular weight poly-L-lysine (30-70 kD) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kD) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kD.  Please check the literature for information on the molecular weight range used for your cell type.

  6. How should I store solutions of poly-lysine?

    Poly-lysine solutions (0.1 to 1 mg/ml) can be sterile-filtered (0.2 um) and should be stored at 2-8 °C.  Solutions should not be stored frozen as thawing can cause a decrease in the size (molecular weight range) of the polymer.

  7. What protocol should I follow for coating Transwells with Product P6407, Poly-D-lysine dihydrochloride?

    The same protocol can be followed for coating plates with poly-lysine.  The transwell membrane is immersed in the poly-lysine solution.

  8. How long can I store poly-lysine coated slides or plates?

    Plates and/or slides can be stored after drying, protected from dust for up to 1 year.  Inspect the plates/slides to make sure there are no cracks in the coating prior to use.  Each lab should determine the maximum length of time for storage based on the application.

  9. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

多聚-L-赖氨酸细胞附着实验方案

使用多聚-赖氨酸将细胞粘附在固相基质上，多聚-赖氨酸可增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用。

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