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P6181

Glu-C Protease

Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8

别名:

V8 蛋白酶

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关于此项目

化学文摘社编号:
66676-43-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
200-350-6
MDL number:
MFCD01324998
EC 号:
3.4.21.19 (BRENDA, IUBMB)
eCl@ss:
32160410
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产品名称

蛋白内切酶 Glu-C 来源于金黄色葡萄球菌  V8 来源于金黄色葡萄球菌 V8, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

Quality Level

200

form

lyophilized powder

analyte chemical class(es)

amino acids

suitability

suitable for protein sequencing

storage temp.

−20°C

Application

来自金黄色葡萄球菌V8的内切蛋白酶Glu-C已被用于：
  • 获得S层蛋白（S-layer protein）的蛋白酶裂解片段（来自嗜热脂肪芽孢杆菌ATCC 12980）以进行亲和力研究。
  • 酶切天然VSTx-3（电压传感器毒素3）肽以测定其序列。
  • 重组纯化PimA（磷脂酰肌醇甘露糖基转移酶）的限制性蛋白水解。
  • 消化糖化血红蛋白，用于同位素稀释液体色谱-串联质谱分析。

Biochem/physiol Actions

来自 Staphylococcus aureus菌株V8的内切蛋白酶Glu-C是一种丝氨酸蛋白酶，能够水解谷氨酸和天冬氨酸残基的羧基端肽键。Glu-C特异性取决于缓冲液和pH以及可能的切割位点周围的结构。在乙酸铵（pH4.0）或碳酸氢铵（pH7.8），酶偏爱切割谷氨酰键，但是，在磷酸缓冲液（pH7.8）中，Glu-C会切割在其他位点。Glu-C被报道在0.2% SDS（十二烷基硫酸纳）和4.0M尿素中有活性。

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000321088
0000301741
0000301741
0000321088
0000248743

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全球贸易项目编号

货号GTIN
P6181-50UG04061835574698