P6181
Glu-C Protease
Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8
别名:
V8 蛋白酶
产品名称
蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8 来源于金黄色葡萄球菌 V8, suitable for protein sequencing, lyophilized powder
grade
Proteomics Grade
form
lyophilized powder
analyte chemical class(es)
amino acids
suitability
suitable for protein sequencing
storage temp.
−20°C
Quality Level
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Application
来自金黄色葡萄球菌V8的内切蛋白酶Glu-C已被用于：
- 获得S层蛋白（S-layer protein）的蛋白酶裂解片段（来自嗜热脂肪芽孢杆菌ATCC 12980）以进行亲和力研究。
- 酶切天然VSTx-3（电压传感器毒素3）肽以测定其序列。
- 重组纯化PimA（磷脂酰肌醇甘露糖基转移酶）的限制性蛋白水解。
- 消化糖化血红蛋白，用于同位素稀释液体色谱-串联质谱分析。
Biochem/physiol Actions
来自 Staphylococcus aureus菌株V8的内切蛋白酶Glu-C是一种丝氨酸蛋白酶，能够水解谷氨酸和天冬氨酸残基的羧基端肽键。Glu-C特异性取决于缓冲液和pH以及可能的切割位点周围的结构。在乙酸铵（pH4.0）或碳酸氢铵（pH7.8），酶偏爱切割谷氨酰键，但是，在磷酸缓冲液（pH7.8）中，Glu-C会切割在其他位点。Glu-C被报道在0.2% SDS（十二烷基硫酸纳）和4.0M尿素中有活性。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
常规特殊物品
此项目有
Two tarantula venom peptides as potent and differential Na(V) channels blockers.
Cherki RS et al.
Toxicon, 77, 58-58 (2014)
Mechanism of acylphosphatase inactivation by Woodward's reagent K.
Paoli, P., et al.
The Biochemical Journal, 855-861, 855-861 (1997)
Kenneth AW
Human Genome Methods (1997)
The abalone egg vitelline envelope receptor for sperm lysin is a giant multivalent molecule.
Swanson, W.J., and Vacquier, V.D.
Proceedings of the National Academy of Sciences of the USA, 94(13), 6724-6729 (1999)
M Jarosch et al.
Microbiology (Reading, England), 146 ( Pt 2), 273-281 (2000-03-09)
The cell surface of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique S-layer lattice. To investigate sequence identities and a common structure-function relationship in S-layer proteins of different B. stearothermophilus wild-type strains, the nucleotide sequence encoding the S-layer
实验方案
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
相关内容
Instructions
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