P5542
磷脂酶 C，磷脂酰肌醇特异性 来源于蜡样芽胞杆菌
lyophilized powder, ≥1,000 units/mg protein (Lowry)
别名:
1-磷脂酰- D - 肌醇肌醇磷酸水解酶，环磷酸盐形成, PI-PLC
产品名称
磷脂酶 C，磷脂酰肌醇特异性 来源于蜡样芽胞杆菌, lyophilized powder, ≥1,000 units/mg protein (Lowry)
form
lyophilized powder
specific activity
≥1,000 units/mg protein (Lowry)
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
storage temp.
−20°C
Quality Level
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
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Analysis Note
根据 Ellman 等人 的研究测定乙酰胆碱酯酶。
Application
蜡状芽孢杆菌的磷脂酰肌醇特异性磷脂酶 C 已被用于从细胞膜上切割绿色荧光蛋白-糖基磷脂酰肌醇 (GFP-GPI) 的 GPI 锚定蛋白。 它也被用于侵袭实验，以测试 PLAP 是否参与了克氏锥虫对人类 2 类型上皮细胞 (HEp-2) 的侵袭。
Biochem/physiol Actions
用于释放锚定的 GPI 膜中的蛋白质。
General description
蜡状芽孢杆菌的磷脂酰肌醇特异性磷脂酶 C (PI-PLC) 是一种 298 残基蛋白。属于一类普遍存在的酶。PI-PLC 具有单一的球状结构域，折叠成八（βα）桶板，其中只有 6 个 βα 单元在管中发现，链 IV 和 V 与 V 和 VI 之间缺少螺旋。
Other Notes
在 30°C、pH 7.4 条件下，每分钟释放 1 个单位的乙酰胆碱酯酶。
Physical form
含有磷酸盐缓冲盐、EDTA 和稳定剂
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
Danielle K Bailey et al.
PloS one, 16(7), e0254794-e0254794 (2021-07-27)
Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia
商品
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
GPI锚定糖蛋白
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