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安全信息

P5381

Sigma-Aldrich

磷酸葡糖异构酶 来源于面包酵母（酿酒酵母）

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

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别名:
D-葡萄糖-6-磷酸酮醇异构酶, PGI, 磷酸糖变位酶
CAS号:
9001-41-6
EC 号:
5.3.1.9 (BRENDA, IUBMB)
EC 号:
232-603-1
MDL编号:
MFCD00131887
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bakers yeast

质量水平

200

类型

Type III

形式

ammonium sulfate suspension

比活

≥400 units/mg protein (biuret)

分子量

tetramer 119,500 Da±600

浓度

≥3 mg/mL protein Biuret

技术

activity assay: suitable

颜色

white

Protein ID登记号

NP_009755.1

UniProt登记号

P12709

储存温度

2-8°C

基因信息

yeast ... PGI1(852495)

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一般描述

研究领域：细胞信号传导

磷酸葡萄糖异构酶是一种普遍存在的多功能细胞质蛋白。该二聚体蛋白的分子量约为119,500 ± 600，由四个几乎相同的亚基组成。

应用

来自面包酵母′（酿酒酵母）的磷酸葡萄糖异构酶的用途：
  • 在血清甘露糖酶法测定中，用于将果糖-6-磷酸转化为葡萄糖-6-磷酸。
  • 作为四酶之一，用于四酶联合反应测定磷酸甘露糖变位酶(PMM)活性 。
  • 在葛根果糖含量测定中，将果糖转化为葡萄糖。

生化/生理作用

磷酸葡萄糖异构酶(PGI)对于D-葡萄糖-6-磷酸和D-果糖-6-磷酸的相互转化至关重要。PGI负责糖酵解的第二步，并参与葡萄糖生成和磷酸戊糖途径。它在细菌和真核生物中高度保守。PGI在烟曲霉(A. fumigatus)存活中起重要作用，在发育、细胞壁葡聚糖生物合成和毒力中发挥多样作用。磷酸葡萄糖异构酶可用作异构酶、神经白细胞素、自分泌运动因子，以及分化和成熟介质。

单位定义

一个酶活性单位是指在pH 7.4、温度25 °C条件下，每分钟将1.0 μm的D-果糖-6-磷酸转化为D-葡萄糖-6-磷酸所需的酶量。

外形

2.6M (NH4)2SO4 中的晶体悬浮液，含 0.1mM 醋酸镁，pH 7.0

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the substrate for Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?

    The substrate for PGI is D-fructose-6-phosphate, according to our Enzyme Assay procedure.

  6. What is the typical protein concentration for Product No. P5381 (PGI)?

    The protein concentration of Product No. P5381 (PGI) can vary from lot-to-lot. It is typically in the range of 5-20 mg protein per mL. The lot-specific concentration will be given on the certificate of analysis.

  7. What is the pH optimum of Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?

    The pH optimum for yeast PGI (Product No. P5381) is 7, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).

  8. What is the KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate, and what does that mean?

    KM is the concentration of substrate that leads to half-maximal velocity. The KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate is 0.167, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
Phosphoglucose Isomerase Is Important for Aspergillus fumigatus Cell Wall Biogenesis
Zhou Y, et al.
mBio, 13(4) (2022)
S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14
Yanagawa T, et al.
Cancer Research, 67(18), 8682-8689 (2007)
Yeast Models of Phosphomannomutase 2 Deficiency, a Congenital Disorder of Glycosylation
Lao JP, et al.
G3 (Bethesda, Md.), 9(2), 413-423 (2019)
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