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经验公式(希尔记法):
C6H6O
化学文摘社编号:
分子量:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
6428463
Grade:
Molecular Biology
Technique(s):
RNA purification: suitable
产品名称
苯酚 溶液, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
SMILES string
Oc1ccccc1
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
grade
Molecular Biology
product line
BioReagent
form
liquid
technique(s)
RNA purification: suitable
pH
4.1-4.5 (25 °C)
storage temp.
2-8°C
Quality Level
Gene Information
human ... GABRA1(2554)
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Application
苯酚溶液可用于:
- 通过标准苯酚-氯仿法提取 RNA
- 细胞壁单糖分析,用以处理鸭跖草(Commelina communisin )叶远轴侧的表皮条
- 作为RNA 分离用裂解变性缓冲液的成分
Biochem/physiol Actions
苯酚溶液可用于多种分子生物学应用,比如标准苯酚-氯仿法核酸提取。这种有机溶剂可用作蛋白变性剂。苯酚-氯仿法是一种广泛使用的核酸提取方法。尽管苯酚不能完全抑制 RNase 活性,但在与氯仿和乙醇联用时,其独特的特性有助于核酸分离。
General description
苯酚溶液是一种在柠檬酸缓冲液中达到饱和的澄清液体。苯酚是羟基直接与芳香碳环键合的有机化合物。一羟基苯的通用名称是苯酚。
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
危险化学品
此项目有
Nucleic Acid Extraction Techniques
Shin JH, et al.
Journal of Separation Science, 209?225-209?225 (2012)
PHENOLS
Smith PWG and Tatchell AR
Aromatic Chemistry, 79-104 (1969)
Sagit Shalel-Levanon et al.
Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)
Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the
mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils
Staudinger BJ, et al.
The Journal of Clinical Investigation, 110(8), 1151-1163 (2002)
Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli
Levanon SS, et al.
Metabolic engineering, 7(5-6), 364-374 (2005)
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