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Merck
CN

P4682

Sigma-Aldrich

苯酚 溶液

BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology

别名:

nucleic acid extraction

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About This Item

经验公式(希尔记法):
C6H6O
CAS号:
分子量:
94.11
Beilstein:
6428463
MDL编号:
UNSPSC代码:
12352104
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

质量水平

产品线

BioReagent

形式

liquid

技术

RNA purification: suitable

pH值(酸碱度)

4.1-4.5 (25 °C)

储存温度

2-8°C

SMILES字符串

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

基因信息

human ... GABRA1(2554)

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一般描述

苯酚溶液是一种在柠檬酸缓冲液中达到饱和的澄清液体。苯酚是羟基直接与芳香碳环键合的有机化合物。一羟基苯的通用名称是苯酚。

应用

苯酚溶液可用于:

  • 通过标准苯酚-氯仿法提取 RNA
  • 细胞壁单糖分析,用以处理鸭跖草(Commelina communisin )叶远轴侧的表皮条
  • 作为RNA 分离用裂解变性缓冲液的成分

生化/生理作用

苯酚溶液可用于多种分子生物学应用,比如标准苯酚-氯仿法核酸提取。这种有机溶剂可用作蛋白变性剂。苯酚-氯仿法是一种广泛使用的核酸提取方法。尽管苯酚不能完全抑制 RNase 活性,但在与氯仿和乙醇联用时,其独特的特性有助于核酸分离。

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

靶器官

Nervous system,Kidney,Liver,Skin

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品

分析证书(COA)

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Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli
Levanon SS, et al.
Metabolic engineering, 7(5-6), 364-374 (2005)
mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils
Staudinger BJ, et al.
The Journal of Clinical Investigation, 110(8), 1151-1163 (2002)
PHENOLS
Smith PWG and Tatchell AR
Aromatic Chemistry, 79-104 (1969)
Sagit Shalel-Levanon et al.
Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)
Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the
Louise Jones et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(20), 11783-11788 (2003-09-18)
Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls

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