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Merck
CN

P4557

Sigma-Aldrich

苯酚 溶液

BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology

别名:

Tris-equilabrated Phenol

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About This Item

经验公式(希尔记法):
C6H6O
CAS号:
分子量:
94.11
Beilstein:
6428463
EC 号:
MDL编号:
UNSPSC代码:
12352104
PubChem化学物质编号:
NACRES:
NA.52
等级:
for molecular biology
方案:
≥99.0% (GC)

等级

for molecular biology

质量水平

产品线

BioReagent

方案

≥99.0% (GC)

表单

liquid

pH值(酸碱度)

7.7-8.1 (25 °C)

储存温度

2-8°C

SMILES字符串

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

基因信息

human ... GABRA1(2554)

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一般描述

Tris 平衡苯酚溶液是一种透明液体,上面覆盖着一层 Tris 缓冲液。 苯酚溶液可用于多种分子生物学应用。苯酚等有机化合物具有芳香族碳环,其上直接连接有羟基。一羟基苯的通用名称是苯酚。

应用

苯酚溶液可用于:

  • 作为消化反应液组分制备不含RNA酶的质粒,用于兔网织红细胞裂解液的体外翻译
  • 从拟南芥莲座叶中提取 DNA
  • 从保存纸(preservation paper)上通过标准苯酚-氯仿法标准提取血液DNA
  • 作为提取缓冲液组分,提取果浆RNA
  • 从根中提取蛋白质
  • 分离粪便 DNA
  • 提取caged RNA

生化/生理作用

苯酚-氯仿法广泛用于核酸提取。尽管苯酚不能完全抑制 RNase 活性,但在与氯仿和乙醇联用时,其独特的特性有助于核酸分离。

组分

  • 1瓶Tris-平衡苯酚(货号P4557)
  • 平衡溶液(货号B5658)

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

靶器官

Nervous system,Kidney,Liver,Skin

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molarity of Phenol solution, Product P4557?

    The Molarity is ~10.6.  The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.

  4. I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?

    The top phase is Tris buffer, so use the lower/bottom phase.

  5. What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?

    This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation.  The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.

  6. What is the final pH of Phenol solution, Product P4557, after adding the buffer?

    The pH is typically 8.0; the specifications state a range of 7.7-8.1.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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