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Merck
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安全信息

P3803

Sigma-Aldrich

苯酚:氯仿:异戊醇25:24:1，10mM Tris饱和溶液，pH8.0，1mM EDTA

for molecular biology

别名:

DNA extraction

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About This Item

UNSPSC代码:
12190000
NACRES:
NA.52

等级

for molecular biology

质量水平

200

检测方案

≥99.0%

形式

liquid

pH值（酸碱度）

6.5-6.9(pH of phenolic phase)

应用

agriculture

储存温度

2-8°C

应用

使用苯酚:氯仿:异戊醇溶液提取，可以从核酸中去除蛋白质。对于核酸粗提混合物，在使用苯酚:氯仿:异戊醇溶液提取前，最好先用一系列蛋白水解酶消解。尽管蛋白质能够被苯酚有效变性，核酸酶活性并未被完全抑制。因此需加入少量异戊醇，进一步确保抑制核酸酶活性。请查阅我们的产品信息表，获得从核酸中去除蛋白质的实验方案。

警告

如果苯酚接触到皮肤，会很快烧伤。因此，使用者需要穿着合适的实验服，包括手套和眼睛护具。如果直接接触到皮肤，立刻用大量水冲洗，并且寻求医学救助。

其他说明

该产品的保质期是两年。

推荐产品

进行DNA提取时，可以用Tris缓冲液平衡，将苯酚相的pH调整到8.0。Sigma提供专门用于DNA提取的P2069产品。该产品包括了平衡缓冲液，能够方便地调节pH值。

相关产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品
易制毒化学品（2类）

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?

    In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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