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Merck
CN

P3303

Asp-N Protease

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
232-642-4
MDL number:
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产品名称

内蛋白酶Asp-N 来源于莓实假单胞菌 突变菌株, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

form

lyophilized powder

analyte chemical class(es)

amino acids

packaging

vial of 2 μg

suitability

suitable for protein sequencing

storage temp.

2-8°C

Quality Level

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Application

来自莓实假单胞菌突变菌株的胞内蛋白酶 Asp-N 已用于特定蛋白质的消化,以制备肽并通过 MS(质谱)法分析生成的肽。

General description

胞内蛋白酶 Asp-N 是一种金属内切蛋白酶。它来自于 莓实假单胞菌 的一个突变菌株,该菌株水解天冬氨酸和半胱氨酸残基 N 端的肽键。 Asp-N 因其对多肽的高度特异性剪切,在蛋白质组学中用于肽图分析和蛋白质序列工作。

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Wilson KJ, et al.
Methods in Protein Sequence Analysis: Proceedings of the 7th International Conference, Berlin, July 3?8, 1988, 310-310 (1988)
T H Jensen et al.
The Journal of biological chemistry, 270(23), 13777-13784 (1995-06-09)
Human immunodeficiency virus encodes the regulatory protein Rev, which is required for expression of viral structural proteins. It binds to an RNA element (RRE) in the viral transcript and up-regulates the cytoplasmic appearance of unspliced and singly spliced viral mRNA.
Sarah Murray et al.
Journal of virology, 80(12), 6171-6176 (2006-05-30)
Adeno-associated virus type 2 (AAV-2) capsid proteins have eight sequence motifs that are potential sites for O- or N-linked glycosylation. Three are in prominent surface locations, close to the sites of cellular receptor attachment and to neutralizing epitopes on or
Functional consequences of preorganized helical structure in the intrinsically disordered cell-cycle inhibitor p27(Kip1).
Bienkiewicz EA, et al.
Biochemistry, 41, 752-752 (2002)
S R Paik et al.
The Biochemical journal, 340 ( Pt 3), 821-828 (1999-06-09)
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies of Alzheimer's disease (AD) and Parkinson's disease respectively. The protein was suggested to provide a possible nucleation centre for plaque formation in AD via selective

实验方案

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

基于优化的 LC-MS/MS 工作流程,通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。

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