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一般描述
胞内蛋白酶 Asp-N 是一种金属内切蛋白酶。它来自于 莓实假单胞菌 的一个突变菌株，该菌株水解天冬氨酸和半胱氨酸残基 N 端的肽键。 Asp-N 因其对多肽的高度特异性剪切，在蛋白质组学中用于肽图分析和蛋白质序列工作。
应用
来自莓实假单胞菌突变菌株的胞内蛋白酶 Asp-N 已用于特定蛋白质的消化，以制备肽并通过 MS（质谱）法分析生成的肽。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
S R Paik et al.
The Biochemical journal, 340 ( Pt 3), 821-828 (1999-06-09)
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Journal of virology, 80(12), 6171-6176 (2006-05-30)
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Atsawasuwan P, et al.
Connective tissue research, 52, 479-479 (2011)
实验方案
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
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