P3201
Platelet-Derived Growth Factor-BB 人
≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
PDGF-BB
产品名称
Platelet-Derived Growth Factor-BB 人, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC and SDS-PAGE)
form
lyophilized powder
potency
1.0-3.0 ng/mL EC50
quality
endotoxin tested
mol wt
~25 kDa
packaging
pkg of 10 μg
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... PDGFB(5155)
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Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
Other Notes
Dimer of the B chain of human PDGF.
Application
Platelet-Derived Growth Factor-BB human has been used :
- As a growth supplement in proximal tubule and glomerular explant cell culture
- In three-dimensional scaffold preparation
- To study the effect of brivanib on growth factor signaling pathways
- In transwell migration assay
Biochem/physiol Actions
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
General description
Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Islet grafting and imaging in a bioengineered intramuscular space
Witkowski P, et al.
Transplantation, 88.9, 1065-1065 (2009)
Anaïs Begemann et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 23(3), 543-554 (2020-11-06)
A few de novo missense variants in the cytoplasmic FMRP-interacting protein 2 (CYFIP2) gene have recently been described as a novel cause of severe intellectual disability, seizures, and hypotonia in 18 individuals, with p.Arg87 substitutions in the majority. We assembled
Structures of a platelet-derived growth factor/propeptide complex and a platelet-derived growth factor/receptor complex.
Shima AHR, et al.
Proceedings of the National Academy of Sciences of the USA, 107.25, 11307-11312 (2010)
Matrix stiffness regulates migration of human lung fibroblasts.
Asano1 S, et al.
Physiological Reports, 5.9, 1-11 (2017)
Yasutoshi Takashima et al.
American journal of physiology. Renal physiology, 310(5), F395-F408 (2016-01-15)
Glomerular integrity and functions are maintained by growth factor signaling. Heparan sulfate, the major component of glomerular extracellular matrixes, modulates growth factor signaling, but its roles in glomerular homeostasis are unknown. We investigated the roles of heparan sulfate 6-O-endosulfatases, sulfatase
商品
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
血小板衍生生长因子是在哺乳动物血清中发现的主要有丝分裂原，在血栓形成过程中从血小板释放。
实验方案
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
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