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安全信息

P2850

Sigma-Aldrich

磷酸酶抑制剂混合物 1

DMSO solution

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EC 号:
200-664-3
UNSPSC代码:
12352200
NACRES:
NA.54

形式

DMSO solution

质量水平

300

运输

wet ice

储存温度

2-8°C

一般描述

磷酸酶抑制剂混合液 1 包括将抑制碱性磷酸酶的 L -同工酶以及丝氨酸/苏氨酸蛋白磷酸酶（如 PP1 和 PP2A）的抑制剂混合物。本品含微囊藻毒素LR、斑蝥素和（−）-p-溴四唑。磷酸酶抑制剂混合液1 已被替换为 P0044。

应用

磷酸酶抑制剂混合液1 已被用于核蛋白分离和电泳迁移率变动分析（EMSA）以激活连环链蛋白。它也已用于蛋白质印迹分析。

生化/生理作用

磷酸酶是许多重要生物过程的活性调节剂。小分子对磷酸酶的抑制有助于研究其生物学作用。

数量

推荐的用法是 100 倍稀释。

分析说明

检测牛肝脏、人胎盘和兔肌肉等动物组织的细胞提取物，以及 A431 和 Jurkat 细胞系的提取物。

WGK

WGK 1

闪点(°F)

188.6 °F

闪点(°C)

87 °C

法规信息

高风险级别生物产品--毒素类产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?

    This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.

  3. At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?

    In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate.

  4. Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?

    Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.

  5. What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?

    Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.

  6. Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast? 

    As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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磷酸酶抑制剂混合物

磷酸酶抑制剂对于所有类型的磷酸化研究都至关重要。对比磷酸酶抑制剂混合物，选择更适合您的研究的产品。

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