一般描述
天然蛋白去糖基化设计用于 PNGase F 抗性天然蛋白 N-连接寡糖的去糖基化。内切糖苷酶 F1、F2 和 F3 对蛋白质构象的敏感性低于 PNGase F，更适用于去除所有类别的 N-连接寡糖而不使蛋白质变性。
用神经氨酸酶、 β-半乳糖苷酶和 N-乙酰氨基葡萄糖苷酶处理糖蛋白，可将所有复杂的寡糖还原为三甘露糖基壳二糖核心。在某些情况下可能需要岩藻糖苷酶。残留的三甘露糖基壳二糖核心结构则可通过内切糖苷酶F3去除。内切糖苷酶F2和F3可即刻分别去除双触角和三触角结构。内切糖苷酶F1可去除低聚甘露糖和杂合结构。
每种类型内切糖苷酶的更多相关信息，请参阅公告。
每种类型内切糖苷酶的更多相关信息，请参阅公告。
应用
天然蛋白去糖基化试剂盒用于各种酶的 N-去糖基化，适用漆酶、 番茄核酸酶TBN1和内切-β-1,3-葡聚糖酶。
储存及稳定性
NDEGLY 套件用湿冰运输，建议储存在2°C至8°C条件下。如按照指示说明储存，则该套件有效期至少为一年。只在环境温度下暴露几天，不会降低该试剂盒中酶的活性。
仅试剂盒组分
产品编号
说明
- Endoglycosidase F1 .3 U
- Endoglycosidase F2 .1 U
- Endoglycosidase F3 .1 U
- Endoglycosidase F1 reaction buffer 200 μL
- Endoglycosidase F2 & 3 reaction buffer 200 μL
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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