usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
input
sample(s) (gel slices)
test parameters
sample volume: 3.5 g agarose
storage temp.
15-25°C
Quality Level
General description
GenElute 凝胶回收试剂盒可用于从标准或低熔点琼脂糖凝胶中快速纯化线性和质粒DNA片段。该试剂盒还可用于从聚丙烯酰胺凝胶中纯化DNA。其典型DNA回收率高达80%。每个纯化柱至多可从多达3.5 g的琼脂糖凝胶中结合10 μg DNA。
Application
GenElute™ 凝胶提取试剂盒已可用于琼脂糖凝胶DNA片段的纯化。
分离所得的DNA适于测序、PCR、限制性酶切、克隆和标记等各种下游应用。
Biochem/physiol Actions
GenElute 凝胶回收试剂盒结合了硅胶结合技术和离心或真空柱形式的便利性。感兴趣的DNA 片段通过溶解琼脂糖凝胶的切片而提取出来。Gel Solubilization Solution可溶解在TBE或TAE缓冲液进行的凝胶反应的琼脂糖凝胶切片。在存在Gel Solubilization Solution的情况下，提取所得的DNA片段选择性吸附到硅胶膜上。经过简单的离心或真空洗涤，去除污染物。最后，在Tris 缓冲液中洗脱结合所得的DNA。
GenElute 凝胶回收试剂盒结合了硅胶结合技术和离心或真空柱形式的便利性。感兴趣的DNA片段可从琼脂糖凝胶切片上提取，并使之结合到硅胶膜上。经过简单的离心或真空洗涤，去除污染物。随后洗脱结合所得的DNA。
纯化所得的DNA适于自动化DNA测序、PCR、限制性酶切、克隆和标记等多种下游应用。
纯化所得的DNA适于自动化DNA测序、PCR、限制性酶切、克隆和标记等多种下游应用。
Features and Benefits
- 可结合多达10 μg DNA
- 回收率高达80%
- 每柱最多可处理3.5 g
- 兼容溶解于TAE 或TBE 缓冲液之中的标准和低熔点琼脂糖。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Gel Solubilization Solution
- Elution Solution (10 mM Tris-HCl, pH 9.0)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3
target_organs
Respiratory system
supp_hazards
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Karen Olsson-Francis et al.
Applied and environmental microbiology, 76(7), 2115-2121 (2010-02-16)
Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria
Prolactin inhibits a major tumor-suppressive function of wild type BRCA1.
Chen KH and Walker Am
Cancer Letters, 375(2), 293-302 (2016)
Margaret A Owegi et al.
The Journal of biological chemistry, 281(40), 30001-30014 (2006-08-08)
Vacuolar proton-translocating ATPase pumps consist of two domains, V(1) and V(o). Subunit d is a component of V(o) located in a central stalk that rotates during catalysis. By generating mutations, we showed that subunit d couples ATP hydrolysis and proton
Vernon K Ward et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(26), 11050-11055 (2007-06-22)
Noroviruses are the major cause of nonbacterial gastroenteritis in humans. These viruses have remained refractory to detailed molecular studies because of the lack of a reverse genetics system coupled to a permissive cell line for targeted genetic manipulation. There is
Attempts to Construct an Enlarged pUC19 via Insertion of HindIII-digested Coliphage ? DNA
Muhamed Amirie
Journal of Experimental Microbiology and Immunology, 18, 96-101 (2014)
实验方案
The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.
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