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Merck
CN

MTOX1004

Sigma-Aldrich

MDR1/BCRP Double Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

别名:

C2BBe1 Cells MDR1/BCRP (-/-/-/-,-/-/-/-)

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.81

product name

MDR1/BCRP Double Knockout Caco-2 Cells, one vial

生物来源

human male colorectal tissue (Source disease: adenocarcinoma)

质量水平

形式

liquid

技术

drug transporter assay: suitable
permeability assay: suitable

OMIM登记号

应用

ADME/TOX

储存温度

−196°C

基因信息

一般描述

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters.

特点和优势

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
  • A functional double knockout of the MDR1 gene and BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP2 substrate
  • The vial format enables the MDR1 and BCRP knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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产品编号
说明
化学品安全说明书

  • MTOX1004MDR1/BCRP Double Knockout Caco-2 Cells, one vial 1 vial化学品安全说明书

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship

商品

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

我们提供了一篇关于肠道外排转运蛋白在药物吸收中作用的文章。

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

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