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一般描述
适于cDNA合成、表达谱及其他需要从超高丰度的rRNA和tRNA中分离mRNA的操作。GenElute mRNA试剂盒为从之前制备的总RNA中分离多腺苷酸化mRNA，或直接从哺乳动物细胞和组织中分离多腺苷酸化mRNA提供了方便的操作方法。直接制备mRNA时，需采用SDS/蛋白酶K降解法破碎细胞或组织，从而释放出RNA并去除RNases。这两种试剂盒均采用与1 μm聚苯乙烯微珠共价偶联的oligo dT30，通过杂交法捕获多腺苷酸化的mRNA。在杂交过程中，聚苯乙烯微珠继续保持悬浮状态，无需进行混合或振荡（这在纤维素或磁微粒方法中较为常见）。oligo(dT) 聚苯乙烯微珠（2-3步）可以采用比常用的oligo(dT) 纤维素微粒法（至少10步）更少的严格洗涤步骤，获得更纯净的mRNA，聚苯乙烯的种类是可选的。采用GenElute试剂盒时，基于mRNA的复合物在一个微量离心滤器上洗涤，然后在10 mM Tris-HCL, pH 7.5.缓冲液洗脱。通过上述任一种试剂盒制备的mRNA均适用于Northern杂交、表达阵列或芯片杂交、cDNA合成、文库构建等广泛的下游应用。
应用
GenElute™ mRNA 小量制备试剂盒已被用于从总 RNA 中纯化 RNA，并分离 RNA。
纯化所得的mRNA可直接用于Northern分析、反转录、PCR、阵列标记及其他常规应用。
特点和优势
- 同时分离RNA、DNA和蛋白质的快速、方便的试剂
- 适合于各种数量规模的组织或细胞，可同时提取多份样本
- 是最高效的总RNA分离方法之一。以新鲜组织或细胞为起始材料，仅需1小时，即可完成纯化
其他说明
更多信息，请见www.sigma-aldrich.com/mrna。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 3
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Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
了解 mRNA 疫苗如何诱导免疫，以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
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