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包装
pkg of 100 μL (library)
pkg of 200 μL (primer 1)
pkg of 200 μL (primer 2)
质量水平
浓度
1 ng/μL (library)
25 μM (primer 1)
25 μM (primer 2)
运输
wet ice
储存温度
−20°C
一般描述
The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.
The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.
The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing
Download deep sequencing data of target ID library.
The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.
The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing
Download deep sequencing data of target ID library.
组分
Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C
法律信息
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
自备
产品编号
说明
价格
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Joop Gäken et al.
Nucleic acids research, 40(10), e75-e75 (2012-02-11)
MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target genes. Due to the incomplete homology of the miRNA to its target site
Shok Ping Lim et al.
Journal of leukocyte biology, 103(5), 855-866 (2018-04-16)
MicroRNAs are short endogenous noncoding RNAs that play pivotal roles in a diverse range of cellular processes. The miR-181 family is important in T cell development, proliferation, and activation. In this study, we have identified BRK1 as a potential target
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