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一般描述
应用
特点和优势
- 基于 ELISA 的格式
- 在 4 个易于遵循的步骤中完成程序
- 提供的所有试剂，包括对照甲基化 DNA
- 检测限为 5 ng 全甲基化 DNA
- 输入 DNA 可能低至 10-200 ng
- 程序在 3.5 小时内完成
- 96 孔格式允许研究单样本或高通量研究
- 对 5-mC 具有高特异性的优化抗体和试剂；对非甲基化胞嘧啶无交叉反应
- 可与贴壁和悬浮细胞、组织、血浆及其他体液如唾液、尿液和血清一起使用
法律信息
相关产品
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
WGK
WGK 3
法规信息
How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
The detection limit of this kit is 5 nanograms of fully methylated DNA.
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
实验方案
Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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