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一般描述
Biofilms are complex structures, which adhere to surfaces and comprise of one or more types of bacteria and fungi. They form an extracellular matrix (ECM) which is generally composed of secreted polymers such as exopolysaccharides (EPS), extracellular DNA (eDNA), peptides and lipids. ExoPolyzyme is a mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids. Each of these enzymes has been previously used to inhibit or remove biofilm. The original development was in collaboration with Scott Tighe and the Extreme Microbiome Project.
应用
ExoPolyzme can be used in biofilm research and as a gentler method for removing the EPS and lipids from the ECM in metagenomic protocols, providing better access to the bacterial cells within the biofilm and eDNA.
特点和优势
- Mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids
- Gently removes the EPS and lipids from the ECM in metagenomic protocols
- Allows better access to the bacterial cells within the biofilm and eDNA
组分
The components in ExoPolyzyme (MBD0064) are:
- α-Amylase
- Cellulase
- β-Glucosidase
- Lyticase
- Alginate lyase
- Lipase
- Hemicellulase
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