用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
neurological disorders
储存温度
room temp
基因信息
human ... ACHE(43)
mouse ... ACHE(11423)
一般描述
乙酰胆碱酯酶（AChEs）是将神经递质乙酰胆碱（ACh）水解为乙酸和胆碱的酶。AChE位于突触间隙并且可通过催化ACh的分解来终止突触传递，从而允许胆碱能神经元在激活后返回静息状态。 它还存在于红细胞、运动和感觉纤维、肌肉、神经以及中央和外周组织的膜中。AChE活性的变化可能是由于暴露于某些杀虫剂环境下导致的，这些杀虫剂起到胆碱酯酶抑制剂的作用。AChE抑制剂也可用于治疗某些疾病，例如痴呆症。
应用
乙酰胆碱酯酶活性测定试剂盒已用于测定大鼠有机磷酸盐模型和脑组织匀浆中乙酰胆碱酯酶的活性 。
特点和优势
兼容高通量的操作系统。 可在比色皿中使用。
适用性
本品适于检测血液、血清和血浆等多种生物样品中的乙酰胆碱酯酶。
原理
该测定是Ellman方法的优化形式，其中由AChE产生的硫代胆碱可与5,5′-二硫代双（2-硝基苯甲酸）反应形成与AChE活性成正比的比色（412nm）产物。一个单位AChE表示在室温，pH7.5条件下，每分钟催化产生.0 μmol硫代胆碱所需的酶量。该试剂盒的线性范围为10-600单位/L AChE活性。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
P Kumari et al.
Parasitology, 144(10), 1412-1416 (2017-06-07)
Demodex canis infestation in dogs remains one of the main challenges in veterinary dermatology. The exact pathogenesis of canine demodicosis is unknown but an aberration in immune status is considered very significant. No studies have underpinned the nexus between induction
Amrita Mukherjee et al.
Environmental science and pollution research international, 26(23), 23442-23452 (2019-06-15)
The current study demonstrates oxidative damage and associated neurotoxicity following pH stress in two freshwater carp Labeo rohita and Cirrhinus cirrhosus. Carp (n = 6, 3 replicates) were exposed to four different pH (5.5, 6, 7.5, and 8) against control (pH 6.8 ± 0.05) for
Marine Droguerre et al.
Frontiers in neuroscience, 14, 563-563 (2020-07-03)
Donepezil (DPZ) is an acetylcholinesterase inhibitor used in Alzheimer's disease to restore cognitive functions but is endowed with limited efficacy. Recent studies pointed out the implication of astroglial networks in cognitive processes, notably via astrocyte connexins (Cxs), proteins involved in
Hemanga Hazarika et al.
Scientific reports, 12(1), 2180-2180 (2022-02-11)
Mosquito-borne infections like dengue, malaria, chikungunya, etc. are a nuisance and can cause profound discomfort to people. Due to the objectional side effects and toxicity associated with synthetic pyrethroids, N,N-diethyl-3-methylbenzamide (DEET), N,N-diethyl phenylacetamide (DEPA), and N,N-di ethyl benzamide (DEBA) based
Study on social isolation as a risk factor in development of Alzheimer?s disease in rats.
Ali A A, et al.
Brain disorders & therapy., 6(230), 2-2 (2017)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门