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Merck
CN

MAK112

Sigma-Aldrich

精氨酸酶活性检测试剂盒

sufficient for 200 colorimetric tests

别名:

Arginase Test Kit

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 200 colorimetric tests

检测方法

colorimetric

相关疾病

cancer; immunological diseases; gastrointestinal diseases

储存温度

−20°C

基因信息

一般描述

精氨酸酶是一种含锰酶,可催化精氨酸转化为尿素和鸟氨酸。大多数哺乳动物体内存在两种同种型精氨酸酶,其组织分布和亚细胞定位有所不同。精氨酸酶I是一种胞质蛋白,主要在肝脏中表达,在肝脏中催化尿素循环的第五步和最后一步。精氨酸酶II是一种线粒体蛋白,组织分布更广,其功能可包括一氧化氮和多胺代谢。精氨酸酶I的活性或表达降低可导致常染色体隐性遗传病高精氨酸血症。血清精氨酸酶活性增加与肝损伤和某些病理状态(如癌症)有关。精氨酸酶还参与免疫系统的多种炎症反应,例如巨噬细胞介导的细胞毒性。

应用

精氨酸酶活性检测试剂盒用于评估精氨酸酶的活性。

特点和优势

兼容高通量的操作系统。

适用性

该试剂盒适用于检测酶制备体系、血清、血浆、组织培养物及类似样品中的精氨酸酶活性。还可用于研究药物对精氨酸酶活性的影响。

原理

在该检测中,精氨酸酶可催化精氨酸转化成尿素和鸟氨酸。产生的尿素与底物特异性反应,产生与存在的精氨酸酶活性成比例的有色产物。

象形图

Health hazardCorrosion

警示用语:

Danger

危险分类

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1A

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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分析证书(COA)

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访问文档库

Razieh Tavakoli Oliaee et al.
Microbial pathogenesis, 126, 368-378 (2018-11-07)
Detection of the mechanism of host/parasite interactions in unresponsive forms of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica is helpful for immunotherapy and vaccine development. In the present study, the gene expression of toll-like receptors (TLRs), TNF-α, iNOS and
Arginase activity, urea, and hydroxyproline concentration are reduced in keratoconus keratocytes.
Stachon T, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 255(1), 91-97 (2017)
Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency.
Martina H.
Journal of inherited metabolic disease, 39(3), 331?340-331?340 (2016)
Melanoma exosomes promote mixed M1 and M2 macrophage polarization.
Bardi G T, et al.
Cytokine, 105 , 63-72 (2018)
Max Hübner et al.
Critical care medicine, 47(8), e700-e709 (2019-06-01)
Cardiopulmonary bypass is associated with severe immune dysfunctions. Particularly, a cardiopulmonary bypass-related long-lasting immunosuppressive state predisposes patients to a higher risk of postoperative complications, such as persistent bacterial infections. This study was conducted to elucidate mechanisms of post-cardiopulmonary bypass immunosuppression.

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