产品名称
多聚酶, lyophilized powder
form
lyophilized powder
technique(s)
DNA extraction: suitable
suitability
suitable for microbiology
application(s)
microbiology
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
元多酶是6种酶的混合物，用于宏基因组学研究中的总DNA分离。 根据S. Tighe设计的最初配方，2元多酶是在与生物分子资源实验室协会(ABRF)宏基因组学和微生物组研究小组(MMRG)商定和合作下评估和开发的。元多酶使用的测试数据可在2017 ABRF MGRG海报上获得。
Biochem/physiol Actions
微生物很难被破坏，因为细胞壁可能形成荚膜或耐药孢子。利用裂解酶（如裂解酶或几丁质酶）可提取 DNA，诱导部分原生质球形成。原生质球随后裂解以释放 DNA。MetaPolyzyme 旨在用于宏基因组研究，如极端和独特环境的微生物和微生物组研究。MetaPolyzyme 旨在通过宏基因组学和元转录组学方法的全基因组鸟枪法测序对微生物进行消化评价。
General description
宏基因组学分析研究直接从给定单个样本（例如环境样本、生物有机体）中分离的所有 DNA。宏基因组学允许调查存在于任何环境（包括极端环境）中，并且在历史上难以分离、培养和研究的微生物。 宏基因组学揭示了新型微生物物种的存在。宏基因组研究的应用包括公共卫生数据、新型蛋白质、酶和天然产物的发现、环境研究和农业调查。
Other Notes
1单位酶在37 °C、pH值为8.0的缓冲液中悬浮，通过600纳米处的浊度检测，每分钟可裂解0.4 μg的藤黄微球菌。
MetaPolyzme 中的酶为：
- 无色肽酶
- 壳质酶
- 溶细胞酶
- 溶葡萄球菌酶
- 溶菌酶
- 变溶菌素
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome.
Maghini DG et al.
Nature Protocols, 16, 458-471 (2021)
Steve Hamner et al.
International journal of environmental research and public health, 16(7), 1097-1097 (2019-04-03)
The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired
Nezar Noor Al-Hebshi et al.
Journal of oral microbiology, 11(1), 1557986-1557986 (2019-01-24)
Studies of the microbiome associated with dental caries have largely relied on 16S rRNA sequence analysis, which is associated with PCR biases, low taxonomic resolution, and inability to accurately study functions. Here, we employed whole metagenome shotgun sequencing, coupled with
Erratum to: The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium Inaugural Meeting Report.
Microbiome, 4(1), 45-45 (2016-08-20)
Maria A Sierra et al.
Environmental microbiome, 17(1), 60-60 (2022-12-22)
Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional
商品
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程：只需加入酶，将试管放入水浴然即可。但这过程中到底发生了什么？
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