product name
MCDB 131培养基, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
形式
powder
浓度
11.6 g/L
技术
cell culture | mammalian: suitable
组分
sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)
运输
ambient
储存温度
2-8°C
一般描述
MCDB-131培养基提供了一个清晰和平衡的营养环境，可选择性地促进特定细胞类型的生长。MCDB-131在补充0.7% FBSP、10 ng/ml表皮生长因子、1 μg/ml氢化可的松后，可支持人微血管内皮细胞（包括人脐静脉内皮细胞）的克隆性生长。MCDB培养基用于特定类型细胞的低蛋白或无血清生长，使用的是激素、生长因子、微量元素或低水平透析胎牛血清蛋白（FBSP）。
应用
MCDB-131培养基已用于：
- 作为牛视网膜内皮细胞基础培养基的组分
- 培养牛肺微血管内皮细胞
- 分离猪颅内内皮细胞和猪主动脉内皮细胞
数量
按照每升培养基11.6克粉末配制。
重悬
补充1.18 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Abdul Jaleel et al.
Molecular and cellular biochemistry, 451(1-2), 1-10 (2018-06-24)
Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally
Kathleen M Job et al.
Anesthesia and analgesia, 123(2), 382-393 (2016-06-23)
The endothelial glycocalyx is an important component of the vascular permeability barrier, forming a scaffold that allows serum proteins to create a gel-like layer on the endothelial surface and transmitting mechanosensing and mechanotransduction information that influences permeability. During acute inflammation
Sandra S Hammer et al.
Diabetologia, 64(7), 1674-1689 (2021-03-27)
Homo sapiens evolved under conditions of intermittent food availability and prolonged fasting between meals. Periods of fasting are important for recovery from meal-induced oxidative and metabolic stress, and tissue repair. Constant high energy-density food availability in present-day society contributes to
G S Ajithkumar et al.
Cardiovascular toxicology, 16(4), 390-405 (2015-12-15)
Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival
Thomas P Burke et al.
eLife, 10 (2021-08-24)
Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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