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Merck
CN
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文件

M8407

Sigma-Aldrich

甲氨蝶呤 水合物

powder, suitable for cell culture, BioReagent

别名:

对-[(2,4-二氨基喋啶-6)-N-甲基甲氨基]苯甲酰谷氨酸 水合物, L-氨甲喋呤 水合物, MTX 水合物, 氟安定 水合物, 氨甲叶酸 水合物, 氨甲喋呤 水合物

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About This Item

经验公式（希尔记法）:
C20H22N8O5 · xH2O
CAS号:
133073-73-1
分子量:
454.44 (anhydrous basis)
MDL编号:
MFCD00150847
UNSPSC代码:
12352207
PubChem化学物质编号:
24897252
NACRES:
NA.75

product name

甲氨蝶呤 水合物, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

产品线

BioReagent

质量水平

200

检测方案

≥98% (HPLC)

形式

powder

技术

cell culture | mammalian: suitable

溶解性

H2O: insoluble

抗生素抗菌谱

neoplastics
parasites

作用机制

enzyme | inhibits

储存温度

−20°C

SMILES字符串

[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O

InChI

1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1

InChI key

FPJYMUQSRFJSEW-ZOWNYOTGSA-N

基因信息

human ... DHFR(1719)

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相关类别

应用

二氢叶酸还原酶的有效抑制剂 和抗肿瘤的研究试剂。 用于抑制二氢叶酸还原酶在DHFR蛋白表达系统中的表达。也显示免疫抑制作用，如类风湿关节炎。
二氢叶酸还原酶的有效抑制剂 和抗肿瘤的研究试剂。 用于抑制二氢叶酸还原酶在DHFR蛋白表达系统中的表达。还可有效治疗耐甲氧嘧啶的 间日 疟原虫。
甲氨蝶呤水合物已用于：
  • 用于球状体形成分析培养物中的二氢叶酸(DHFR)抑制剂
  • 用作杜氏改良Eagles培养基（DMEM）组分，筛选转染细胞
  • 活力和共培养分析

生化/生理作用

甲氨蝶呤是叶酸拮抗剂。
甲氨蝶呤被用于抑制二羟叶酸还原酶，并选择DHFR过表达。

相关产品

产品编号
说明
价格

象形图

Skull and crossbonesHealth hazard
GHS06,GHS08

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I make a solution of Methotrexate hydrate, Product M8407?

    Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.

  4. How do I store a stock solution of Methotrexate hydrate, Product M8407?

    A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.

  5. How do I sterilize a solution of Methotrexate hydrate, Product M8407?

    A solution of methotrexate can be sterile filtered using a 0.22 mm filter

  6. How often does Methotrexate hydrate, Product M8407, need to be added to the media during selection?

    Fresh methotrexate should be added each time the culture media is changed.  Regardless of cell growth, most media will need to be changed at least once a week.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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