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M7027

Sigma-Aldrich

甲基纤维素

viscosity: 15 cP, BioReagent, suitable for cell culture

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About This Item

CAS号:
9004-67-5
MDL编号:
MFCD00081763
UNSPSC代码:
12352201
PubChem化学物质编号:
24897135
NACRES:
NA.75

生物来源

synthetic (organic)

质量水平

200

产品线

BioReagent

表单

powder

技术

cell culture | mammalian: suitable

粘度

12-18 cP, 2 % in H2O(20 °C)

溶解性

water: 50 mg/mL, clear to hazy

SMILES字符串

[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]

InChI key

YLGXILFCIXHCMC-JHGZEJCSSA-N

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一般描述

甲基纤维素由纤维素醚化而得。这种醚类纤维素商品具有两亲性及原本的理化特性。甲基纤维素抗酶解、抗消化、无过敏性、无毒性。

应用

甲基纤维素可用于制备半固体基质, 用作细胞培养凝胶，用于培养集落形成细胞 （如CFU-G、CFU-GM）和用于毒性检测的其他细胞。粘度低，不适用于噬菌斑检测或克隆。
甲基纤维素可用于：
  • 在经口管饲法中作为药物二甲双胍的溶剂
  • 在乳腺球培养中，作为乳腺上皮细胞生长培养基的组分
  • 在悬滴法球状体侵袭试验中，作为球状体三维共培养补充剂

生化/生理作用

甲基纤维素广泛应用多个行业，在食品业中可用作乳化剂、质感剂、增稠剂和胶凝添加剂。甲基纤维素也可用于各种化妆品和个人护理用品，例如洗发水、头发造型用品、液体皂、沐浴露、乳霜、牙膏，使稠度均匀。此外，它也可作为药物添加剂，生产营养补充剂胶囊。甲基纤维素可替代冻存液中的甘油。它可与培养基添加剂共同作用，增强多系或单系造血祖细胞的增殖和分化。这种纤维素衍生物还可用于防止多种细胞的粘附能力和细胞功能丧失，包括人皮肤成纤维细胞、人黑色素细胞和小鼠肺成纤维细胞悬液。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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分析证书(COA)

Lot/Batch Number
0000409636
0000395386
0000395387
0000363774
0000365187

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I make a solution of Methyl cellulose, Product M7027?

    There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.

  4. Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?

    Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media.  Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.

  5. How do I sterilize a solution of Methyl cellulose, Product M7027?

    A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to chunks of methylcellulose falling out of solution.

  6. What concentration of Methyl cellulose, Product M7027, is used for cell culture?

    Product No. M7027 is used at approximately 50 g/L in media to enhance viscosity of culture media.

  7. Is Methyl cellulose, Product M7027, the same as Methocel?

    Methocel is a brand name and is a synonym for methylcellulose.

  8. How do I store a stock solution of Methyl cellulose, Product M7027?

    A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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