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Merck
CN

M5524

Sigma-Aldrich

Murashige & Skoog 基础盐混合物 (MS)

powder, suitable for plant cell culture

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别名:
MS Basal Salts, MS0 Basal Salts, MSO Basal Salts
UNSPSC代码:
12352207
NACRES:
NA.72

形式

powder

质量水平

技术

cell culture | plant: suitable

应用

agriculture

运输

ambient

储存温度

2-8°C

一般描述

Murashige & Skoog培养基广泛用于植物组织生长培养基。 M&S基础培养基含有大量营养素,包括高含量的硝酸盐和有机添加剂,如琼脂、糖、维生素和生长调节剂。 经常添加到M&S中的重要生长调节剂包括IAA(生长素/形态发生素)和激动素(细胞分裂素/细胞分裂促进剂)。

应用

Murashige & Skoog 基础盐混合物(MS)已用于维持转化的烟草细胞的生长和再生。它也可用作拟南芥 (Col-0)种子发芽的生长培养基。

公式变量

含有Linsmaier 和 Skoog(1962)所述的大量和微量营养素。

培养基配方

数量

配制成每升培养基含 4.3 克粉末。

制备说明

Murashige & Skoog培养基可以由粉末复溶,或者将作为M&S完全培养基主要成分(大量营养素混合物和维生素混合物)的产品混合后复溶。 Murashige & Skoog盐混合物(M5524)包含原始经典配方中的大量营养素和微量营养素。它可以与M&S维生素或Gamborg维生素结合,辅以蔗糖、琼脂、生长素(IAA)和细胞分裂素(激动素),形成植物组织生长完全培养基。

其他说明

储存
粉末培养基极易吸湿,必须防止空气中的水分对其潮解。单个包装的全部粉末应尽可能在打开后立即使用。

将干燥培养基保存在0-5°C的干燥器中。

粉末培养基变质可以通过以下方式识别:1)颜色变化;2)粉末中的颗粒化、结块或颗粒物;3)不溶性;4)pH变化;或5)正确使用时无法促进生长。

培养基制备

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危险声明

危险分类

Eye Irrit. 2 - Ox. Sol. 3

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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