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安全信息

M5519

Sigma-Aldrich

Murashige & Skoog 基本培养基

powder, suitable for plant cell culture

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别名:
MS 基本培养基
MDL编号:
MFCD00240976
UNSPSC代码:
12352207
NACRES:
NA.72

形式

powder

质量水平

200

技术

cell culture | plant: suitable

应用

agriculture

储存温度

2-8°C

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相关类别

一般描述

Murashige & Skoog培养基广泛用于植物组织生长培养基。 M&S基础培养基含有大量营养素，包括高含量的硝酸盐和有机添加剂，如琼脂、糖、维生素和生长调节剂。 经常添加到M&S中的重要生长调节剂包括IAA（生长素/形态发生素）和激动素（细胞分裂素/细胞分裂促进剂）。

应用

Murashige & Skoog培养基可用于转化研究用植物组织培养基。

公式变量

含有Linsmaier 和 Skoog（1962）所述的大量、微量营养素和维生素。

培养基配方

数量

配制成每升培养基含 4.4 克粉末。

制备说明

Murashige & Skoog培养基可以由粉末复溶，或者将作为M&S完全培养基主要成分（大量营养素混合物和维生素混合物）的产品混合后复溶。Murashige & Skoog培养基（M5519）包含原始经典配方中的微量营养素和维生素。 它可以添加蔗糖、琼脂、生长素（IAA）和细胞分裂素（激动素），形成植物组织生长完全培养基。

相关产品

产品编号
说明
价格

象形图

Flame over circleExclamation mark
GHS03,GHS07

警示用语：

Warning

危险声明

H272,H319

危险分类

Eye Irrit. 2 - Ox. Sol. 3

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    It is media containing the macro- and micronutrients, and vitamins.

  4. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    Product M5519 is the complete medium whereas M5524 is just the basal salt medium.

  5. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    When M5519 is on backorder, you can purchase Murashige and Skoog Basal Salt Mixture (Product No. M5524) and Murashige and Skoog Vitamin Powder (Product No. M7150). You will use 4.3 g/L of M5524 (rather than 4.4 g/L of M5519) and 1 mL/L of M7150. When combined, the salt and organic content is the same as Product No. M5519.

  6. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    This product does not contain any agar.

  7. Product M5519, Murashige and Skoog Basal Medium, has precipitates; will that affect the growth?

    Precipitates are known to occur, with time, in plant tissue culture media.  The precipitates have been analyzed.  They are composed of small pale yellow-white particles.  Analysis of precipitates indicated a predominance of iron, phosphate, and zinc.  The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions.  When the solubility of ferric phosphate is exceeded precipitation occurs.  There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.

  8. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    We would not suggest autoclaving the media, because a precipitate may form.  It is advisable to add pre-autoclaved water as it contains less oxygen; this reduces the likelihood of oxidation of ferrous to ferric ions.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Ajay Kumar et al.
BMC genomics, 13, 597-597 (2012-11-07)
Development of a high quality reference sequence is a daunting task in crops like wheat with large (~17Gb), highly repetitive (>80%) and polyploid genome. To achieve complete sequence assembly of such genomes, development of a high quality physical map is
Pei-Feng Sun et al.
PloS one, 9(12), e114196-e114196 (2014-12-03)
Yeasts are widely distributed in nature and exist in association with other microorganisms as normal inhabitants of soil, vegetation, and aqueous environments. In this study, 12 yeast strains were enriched and isolated from leaf samples of the carnivorous plant Drosera
Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
Sina Fischer et al.
Journal of experimental botany, 72(2), 415-425 (2020-10-11)
High Arsenic Concentration 1 (HAC1), an Arabidopsis thaliana arsenate reductase, plays a key role in arsenate [As(V)] tolerance. Through conversion of As(V) to arsenite [As(III)], HAC1 enables As(III) export from roots, and restricts translocation of As(V) to shoots. To probe
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