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质量水平
无菌性
0.2 μm filtered
形式
lyophilized powder
比活
≥4000 units/mg protein (biuret)
分子量
23 kDa
运输
wet ice
储存温度
−20°C
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一般描述
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成，可能是一种有效的龋病防治剂。
应用
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
生化/生理作用
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
单位定义
以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
外形
含聚蔗糖和琥珀酸钠缓冲盐的冻干粉
制备说明
由 M 9901 制备
其他说明
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Carli B Jones et al.
Frontiers in genetics, 13, 799615-799615 (2022-04-19)
Short read 16 S rRNA amplicon sequencing is a common technique used in microbiome research. However, inaccuracies in estimated bacterial community composition can occur due to amplification bias of the targeted hypervariable region. A potential solution is to sequence and assess
John M Pfeffer et al.
Journal of bacteriology, 188(3), 902-908 (2006-01-24)
The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E.
Composition of gastrointestinal microbiota in association with treatment response in individuals with metastatic castrate resistant prostate cancer progressing on enzalutamide and initiating treatment with anti-PD-1 (pembrolizumab).
Peiffer, et al.
Neoplasia, 32, 100822-100822 (2022)
Tom Vanhoutte et al.
FEMS microbiology ecology, 48(3), 437-446 (2004-06-01)
According to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. In this study, the temporal stability of both the predominant population and
A rapid and efficient method of lysis of Listeria and other gram-positive bacteria using mutanolysin
Fliss, I., et al.
Biotechniques, 11, 456-457 (1991)
实验方案
在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
This procedure may be used for Mutanolysin products.
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