M4782
变溶菌素 来源于球孢链霉菌 ATCC 21553
0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)
产品名称
变溶菌素 来源于球孢链霉菌 ATCC 21553, 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)
sterility
0.2 μm filtered
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
Biochem/physiol Actions
变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
General description
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成，可能是一种有效的龋病防治剂。
Other Notes
以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
Physical form
含聚蔗糖和琥珀酸钠缓冲盐的冻干粉
Preparation Note
由 M 9901 制备
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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Fliss, I., et al.
Biotechniques, 11, 456-457 (1991)
Tom Vanhoutte et al.
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实验方案
This procedure may be used for Mutanolysin products.
在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
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