Quality Level
sterility
0.2 μm filtered
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
shipped in
wet ice
storage temp.
−20°C
General description
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成,可能是一种有效的龋病防治剂。
Application
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
Biochem/physiol Actions
变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶,可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。
Physical form
含聚蔗糖和琥珀酸钠缓冲盐的冻干粉
Preparation Note
由 M 9901 制备
Other Notes
以粪链球菌细胞壁混悬液为底物,在1mL体积中,1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
查看更多关于 复合糖分析用酶的信息, 请访问 www.sigma-aldrich.com/enzymeexplorer
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Explore all of our products under 变溶菌素 来源于球孢链霉菌 ATCC 21553
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
This procedure may be used for Mutanolysin products.
在测定变溶菌素活性时,使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
相关内容
Instructions
Tom Vanhoutte et al.
FEMS microbiology ecology, 48(3), 437-446 (2004-06-01)
According to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. In this study, the temporal stability of both the predominant population and
A rapid and efficient method of lysis of Listeria and other gram-positive bacteria using mutanolysin
Fliss, I., et al.
Biotechniques, 11, 456-457 (1991)
John M Pfeffer et al.
Journal of bacteriology, 188(3), 902-908 (2006-01-24)
The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M4782-5KU | 04061834059226 |
