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安全信息

M4526

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, sterile liquid, suitable for cell culture

别名:

αMEM, MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

product name

Eagle最低必需培养基, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

应用

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞（MSC）
  • 小鼠成骨前细胞系

重悬

添加 0.292g/L L-谷氨酰胺。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Zhourui Ma et al.
Journal of molecular histology, 51(3), 241-250 (2020-05-14)
Using a large-scale quantitative mesenchymal stem cells (MSCs) membrane proteomics analysis, we identified a large group of ciliary proteins in the MSCs membrane fraction, which prompted us to examine the cilia, intricate organelles that were originally discovered approximately 100 years ago.
Jason M Lajoie et al.
Protein engineering, design & selection : PEDS, 32(5), 219-230 (2019-11-27)
Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening
Michelle L Churchman et al.
Cancer cell, 33(5), 937-948 (2018-04-24)
Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
Philipp von Eisenhart-Rothe et al.
Investigative ophthalmology & visual science, 59(12), 5082-5097 (2018-10-30)
Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized by accumulation of intracellular waste. Cln6nclf mice have a naturally occurring mutation in ceroid-lipofuscinosis neuronal (CLN) protein 6 and are a
Nicolás E Muzzio et al.
Materials science & engineering. C, Materials for biological applications, 80, 677-687 (2017-09-04)
The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from
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