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安全信息

M4526

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

别名:

αMEM, MEM

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选择尺寸

500 ML
¥549.68

¥549.68

目录价¥732.90节省 25%

国内现货，预计发货时间2025年4月27日详情

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选择尺寸

变更视图
500 ML
¥549.68

About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

¥549.68

目录价¥732.90节省 25%

国内现货，预计发货时间2025年4月27日详情

获取大包装报价

产品名称

Eagle最低必需培养基, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

运输

ambient

储存温度

2-8°C

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此商品
M8042M5650M0644
Sigma-Aldrich

M4526

Eagle最低必需培养基

Sigma-Aldrich

M8042

Eagle最低必需培养基

Sigma-Aldrich

M5650

Eagle最低必需培养基

Sigma-Aldrich

M0644

Eagle最低必需培养基

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

应用

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物[1]
  • 人类间充质干细胞（MSC）[2]
  • 小鼠成骨前细胞系[3]

重悬

添加 0.292g/L L-谷氨酰胺。

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说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    RNBM0562
    RNBL7706
    RNBL2659
    RNBL5431
    RNBL0707

    没有发现合适的版本？

    如果您需要特殊版本，可通过批号或批次号查找具体证书。

    搜索COA

    已有该产品？

    在文件库中查找您最近购买产品的文档。

    访问文档库

    Jason M Lajoie et al.
    Protein engineering, design & selection : PEDS, 32(5), 219-230 (2019-11-27)
    Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening
    Zhourui Ma et al.
    Journal of molecular histology, 51(3), 241-250 (2020-05-14)
    Using a large-scale quantitative mesenchymal stem cells (MSCs) membrane proteomics analysis, we identified a large group of ciliary proteins in the MSCs membrane fraction, which prompted us to examine the cilia, intricate organelles that were originally discovered approximately 100 years ago.
    Michelle L Churchman et al.
    Cancer cell, 33(5), 937-948 (2018-04-24)
    Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
    Philipp von Eisenhart-Rothe et al.
    Investigative ophthalmology & visual science, 59(12), 5082-5097 (2018-10-30)
    Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized by accumulation of intracellular waste. Cln6nclf mice have a naturally occurring mutation in ceroid-lipofuscinosis neuronal (CLN) protein 6 and are a
    Ricardo Rodrigues-Pinto et al.
    Scientific reports, 8(1), 12866-12866 (2018-08-29)
    The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is
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