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安全信息

M2279

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

product name

Eagle最低必需培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

最低必需培养基（MEM）是使用最广泛的合成细胞培养基之一。它包含更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。MEM已用于在胎牛血清（FBS）、小牛或马血清环境中培养各种单层细胞和附着细胞系（如成纤维细胞）。

应用

Eagle最低必需培养基已用于培养：
  • 主动脉瓣小叶的外植体
  • 人肝癌细胞系（HepG2）细胞
  • 来源于人胎儿大脑祖细胞的星形胶质细胞（PDA）
  • 乳腺癌细胞系(MCF-7)
  • 人成胶质细胞瘤细胞系(T98G)

制备说明

补充0.292 g/L L-谷氨酰胺。

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说明
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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Qingyun Li et al.
Neuron, 101(2), 207-223 (2019-01-05)
Microglia are increasingly recognized for their major contributions during brain development and neurodegenerative disease. It is currently unknown whether these functions are carried out by subsets of microglia during different stages of development and adulthood or within specific brain regions. Here
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Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)
Kaisa-Emilia Makkonen et al.
Journal of virology, 87(20), 11148-11159 (2013-08-09)
Baculoviruses are insect-specific viruses commonly found in nature. They are not able to replicate in mammalian cells but can transduce them when equipped with an appropriate mammalian cell active expression cassette. Although the viruses have been studied in several types
Emily R Trzeciak et al.
Cells, 11(6) (2022-03-26)
The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T
Chiara Vidoni et al.
Neurochemistry international, 117, 174-187 (2017-05-24)
Parkinsonian-like motor deficits in Huntington's Disease (HD) patients are associated with abnormal dopamine neurotransmission in the striatum. Dopamine metabolism leads to the formation of oxidized dopamine quinones that exacerbates mitochondrial dysfunction with production of reactive oxygen species (ROS) that eventually
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