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Merck
CN

L8402

Sigma-Aldrich

溶菌酶 来源于人类中性粒细胞

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

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别名:
粘肽N-乙酰胞壁质水解酶, 胞壁质酶
CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

human neutrophils

质量水平

重组

expressed in E. coli

检测方案

≥95% (SDS-PAGE)

形式

lyophilized powder

比活

≥100,000 units/mg protein (E1%/280)

技术

cell based assay: suitable

适用性

suitable for cell lysis

UniProt登记号

应用

cell analysis

储存温度

−20°C

InChI

1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1

InChI key

ZJCXKXFAOCLSRV-RYLVIXIQSA-N

基因信息

human ... LYZ(4069)

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一般描述

溶菌酶大量存在于动植物界。它是一种天然食品防腐剂,也存在于特定的细菌细胞壁中。溶菌酶通常存在于泪液、乳汁、尿液和唾液中。

应用

人中性粒细胞溶菌酶已被用于棋盘法,以建立拟肽 1 与免疫效应分子之间的协同抗大肠杆菌

生化/生理作用

溶菌酶参与防御机制。具有通过在底物中产生空间应力刺激催化的能力。

单位定义

在 2.6 mL 反应混合液(1 cm 光路)中,使用溶壁微球菌悬浮液作为底物,在 25 ℃、pH 6.24 条件下,1 个单位将产生 0.001/min 的 Δa450

外形

从含 100 mM NaCl 的 50 mM 乙酸钠 (pH 6.0) 冻干

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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María Victoria Humbert et al.
PLoS pathogens, 13(6), e1006448-e1006448 (2017-07-01)
Pathogenic and commensal Neisseria species produce an Adhesin Complex Protein, which was first characterised in Neisseria meningitidis (Nm) as a novel surface-exposed adhesin with vaccine potential. In the current study, the crystal structure of a recombinant (r)Nm-ACP Type I protein
Selectivity in the potentiation of antibacterial activity of alpha-peptide beta-peptoid peptidomimetics and antimicrobial peptides by human blood plasma
Hein-Kristensen L, et al.
Research in Microbiology, 164(9), 933-940 (2013)
Maj Hedtjärn et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(12), 1333-1351 (2004-12-31)
Brain ischemia triggers an inflammatory reaction that progresses for days to weeks and seems to have a role in secondary progression of injury. Inflammation induces a complex pattern of signaling molecules with partly contradictory actions, and the responses may be
Natural Food Antimicrobial Systems (2000)
Thomas Gibaud et al.
Physical review letters, 110(5), 058303-058303 (2013-02-19)
We show that gels formed by arrested spinodal decomposition of protein solutions exhibit elastic properties in two distinct frequency domains, both elastic moduli exhibiting a remarkably strong dependence on volume fraction. Considering the large difference between the protein size and

实验方案

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

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