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生物来源
human neutrophils
质量水平
重组
expressed in E. coli
方案
≥95% (SDS-PAGE)
表单
lyophilized powder
比活
≥100,000 units/mg protein (E1%/280)
技术
cell based assay: suitable
适用性
suitable for cell lysis
UniProt登记号
应用
cell analysis
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一般描述
溶菌酶大量存在于动植物界。它是一种天然食品防腐剂,也存在于特定的细菌细胞壁中。溶菌酶通常存在于泪液、乳汁、尿液和唾液中。
应用
人中性粒细胞溶菌酶已被用于棋盘法,以建立拟肽 1 与免疫效应分子之间的协同抗大肠杆菌。
生化/生理作用
溶菌酶参与防御机制。具有通过在底物中产生空间应力刺激催化的能力。
单位定义
在 2.6 mL 反应混合液(1 cm 光路)中,使用溶壁微球菌悬浮液作为底物,在 25 ℃、pH 6.24 条件下,1 个单位将产生 0.001/min 的 Δa450。
外形
从含 100 mM NaCl 的 50 mM 乙酸钠 (pH 6.0) 冻干
其他说明
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Natural Food Antimicrobial Systems (2000)
Maj Hedtjärn et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(12), 1333-1351 (2004-12-31)
Brain ischemia triggers an inflammatory reaction that progresses for days to weeks and seems to have a role in secondary progression of injury. Inflammation induces a complex pattern of signaling molecules with partly contradictory actions, and the responses may be
Structure of the Neisseria Adhesin Complex Protein (ACP) and its role as a novel lysozyme inhibitor.
María Victoria Humbert et al.
PLoS pathogens, 13(6), e1006448-e1006448 (2017-07-01)
Pathogenic and commensal Neisseria species produce an Adhesin Complex Protein, which was first characterised in Neisseria meningitidis (Nm) as a novel surface-exposed adhesin with vaccine potential. In the current study, the crystal structure of a recombinant (r)Nm-ACP Type I protein
Selectivity in the potentiation of antibacterial activity of alpha-peptide beta-peptoid peptidomimetics and antimicrobial peptides by human blood plasma
Hein-Kristensen L, et al.
Research in Microbiology, 164(9), 933-940 (2013)
Anne Derbise et al.
The Journal of infectious diseases, 207(10), 1535-1543 (2013-02-14)
Yersinia pestis (the plague bacillus) and its ancestor, Yersinia pseudotuberculosis (which causes self-limited bowel disease), encode putative homologues of the periplasmic lysozyme inhibitor Ivy and the membrane-bound lysozyme inhibitor MliC. The involvement of both inhibitors in virulence remains subject to
实验方案
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
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