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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
235-747-3
MDL number:
EC 号:
grade
Molecular Biology
form
lyophilized powder
specific activity
≥40,000 units/mg protein
mol wt
single-chain 14.3 kDa
composition
protein, ≥90%
UniProt accession no.
storage temp.
−20°C
Gene Information
chicken ... LYZ(396218)
Application
酶可分解细菌的细胞壁;用于制备原生质体。
Lysozyme hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine, a polysaccharide backbone of peptidoglycans in the cell wall structure of many microorganisms. This is particularly useful for lysing Gram-positive and Gram-negative bacteria for subsequent nucleic acid extraction.
Biochem/physiol Actions
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D -氨基葡萄糖残基之间以及壳糊精中N-乙酰-D -氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。
该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。
该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D -氨基葡萄糖残基之间以及壳糊精中N-乙酰-D -氨基葡萄糖残基之间的β(1→4)连接。革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。
Features and Benefits
- Highly purified by repeated crystallization and dialysis
- Each lot is use-tested for isolation of plasmid DNA from E. coli
Physical form
Lyophilized powder, essentially salt-free
Preparation Note
3 次结晶
Other Notes
One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
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警示词
Danger
危险代码
预防性声明
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
怎么回事?
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
PPE(个人防护设备)
Eyeshields, Gloves, type N95 (US)
